| Product Description | Aladdin's Deacetylase Inhibitor Cocktail, 100X is designed to maintain the acetylation of histones and other non-histone proteins. It contains 40μM Trichostatin A, 1mM EX-527, 400mM nicotinamide, and 200mM sodium butyrate in 70% DMSO.Cell or tissue extracts contain many endogenous proteases, phosphatases, and deacetylases, which easily leads to degradation or de-modification of protein samples. Therefore, adding appropriate inhibitors is generally necessary for protein extraction. To better detect the acetylation level of intracellular proteins, appropriate deacetylase inhibitors should be added to the cell culture for a period of time before collecting cells for protein extraction. This product can effectively inhibit various deacetylases including class I/II/III HDAC and maintain the acetylation state of proteins.For the protein extraction from different types of samples, aladdin provides a wide variety of protease, phosphatase, deacetylase and other inhibitor cocktails for selection. Please refer to Table 1 in Appendix for details.This product can effectively inhibit various deacetylases, including class I/II/III deacetylases. Among its components, Trichostatin A is a reversible inhibitor of class I/II deacetylase. EX-527 is a selective and reversible inhibitor of SIRT1 and also has a certain inhibitory effect on SIRT2 and SIRT3. Nicotinamide is a reversible inhibitor of Class III deacetylase (Sirtuins, SIRT1-7). Sodium butyrate is a reversible inhibitor of class I/II deacetylase. Please refer to Table 2 in Appendix for specific information of various inhibitors.This product is usually sufficient for preparing 100ml of lysis buffer or cell culture medium.
Precautions： It is recommended to store this product in aliquot. This product should be added into solutions immediately before use.The deacetylase inhibitors contained in this product are harmful to human. Please take effective measures to avoid direct contact or inhalation.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Appendix：Table 1. Inhibitor cocktails from .Cat. No.Product NamePack SizeP1005Protease Inhibitor Cocktail for General Use, 100X1mlP1006Protease Inhibitor Cocktail for General Use, 100X5mlP1008Protease inhibitor cocktail for general use, MS-SAFE, 50X1mlP1009Protease inhibitor cocktail for general use, MS-SAFE, 50X5mlP1010Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X1mlP1011Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X5mlP1015Protease inhibitor cocktail for plant cell and tissue extracts, 100X1mlP1016Protease inhibitor cocktail for plant cell and tissue extracts, 100X5mlP1020Protease inhibitor cocktail for fungal and yeast extracts, 100X1mlP1021Protease inhibitor cocktail for fungal and yeast extracts, 100X5mlP1025Protease inhibitor cocktail for bacterial cell extracts, 100X1mlP1026Protease inhibitor cocktail for bacterial cell extracts, 100X5mlP1030Protease inhibitor cocktail for purification of His-tagged proteins, 100X1mlP1031Protease inhibitor cocktail for purification of His-tagged proteins, 100X5mlP1045Protease and phosphatase inhibitor cocktail for general use, 50X2ml eachP1046Protease and phosphatase inhibitor cocktail for general use, 50X10ml eachP1048Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X1ml eachP1049Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X5ml eachP1050Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X2ml eachP1051Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X10ml eachP1055Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X2ml eachP1056Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X10ml eachP1060Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X2ml eachP1061Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X10ml eachP1065Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X2ml eachP1066Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X10ml eachP1081Phosphatase inhibitor cocktail A, 50X2mlP1082Phosphatase inhibitor cocktail A, 50X10mlP1086Phosphatase inhibitor cocktail B ,50X2mlP1087Phosphatase inhibitor cocktail B ,50X10mlP1091Phosphatase inhibitor cocktail C ,50X2mlP1092Phosphatase inhibitor cocktail C ,50X10mlP1096Phosphatase inhibitor cocktail D ,50X2mlP1097Phosphatase inhibitor cocktail D ,50X10mlP1112Deacetylase Inhibitor Cocktail,100X1mlP1113Deacetylase Inhibitor Cocktail,100X5mlTable 2. Information of commonly used protease, phosphatase, and deacetylase inhibitors. NameMW Target EnzymeInhibition AEBSF239.5Serine proteaseirreversibleAprotinin6511.5Serine proteaseReversibleBestatin308.38AminopeptidaseReversibleE64357.4Cysteine proteaseirreversibleLeupeptin475.6Serine and Cysteine proteaseReversiblePepstatin A685.9Aspartic proteaseReversibleEDTA372.24MetalloproteinaseReversiblePhenanthroline198.22MetalloproteinaseReversiblePhosphoramidon587.5MetalloproteinaseReversibleSodium fluoride41.99Acid phosphataseReversibleSodium pyrophosphate221.9Serine/Threonine Phosphataseirreversibleβ-glycerophosphate306.11Serine/Threonine PhosphataseReversibleImidazole68.2Alkaline phosphataseReversibleSodium molybdate205.9Acid phosphataseirreversibleSodium tartrate 230.1Acid phosphataseReversibleSodium orthovanadate183.91Alkaline and Tyrosine PhosphatasesReversible(-)-p-Bromotetramisole373.22Alkaline phosphataseirreversibleCantharidin196.20Protein Phosphatase 2AReversibleMicrocystin-LR995.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleCalyculin A1009.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleTrichostatin A302.37class I/II HDACReversibleEX-527248.71SIRT1 over SIRT2 & SIRT3ReversibleNicotinamide122.12class III HDACReversibleSodium butyrate110.09class I/II HDACReversible
Instructions for Use： 1. This product is a 100X concentrated stock solution and should be added to the lysis buffer or culture medium at 1:100 before use (e.g., add 10μl of deacetylase inhibitor cocktail to 1ml of lysis buffer). The lysis buffer or culture medium containing the deacetylase inhibitors should be prepared freshly. After preparation, protein extraction from cells or tissues should be performed immediately.
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