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Denosumab (anti-TNFSF11) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to RANKL, >95%, high purity, Human IgG1, INHIBITOR of Tumor necrosis factor ligand superfamily member 11 inhibitor

  • Azide Free
  • Carrier Free
  • ExactAb™
  • Low Endotoxin
  • Recombinant
  • Validated
  • ≥95%(SDS-PAGE&SEC)
  • Lot by Lot
Features and benefits
  • Short Overview:

    Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

  • Application: ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
  • Species reactivity(Reacts with): Cynomolgus monkey,Human
  • Isotype: Human IgG1
    Application:
  • Animal Model
  • ELISA
  • Flow Cytometry
  • Functional Assay
Item Number
Ab169305
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab169305-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab169305-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab169305-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$627.90
Ab169305-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$987.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

Basic Description

Product NameDenosumab (anti-TNFSF11) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to RANKL, >95%, high purity, Human IgG1
SynonymsDenosumab; AMG-162; Prolia; Xgeva; Immunoglobulin G2; Ranmark; CD254 antibody; hRANKL2 antibody; ODF antibody; OPGL antibody; OPTB2 antibody; Osteoclast differentiation factor antibody; Osteoprotegerin ligand antibody; Rank Ligand antibody; RANKL antibody
Specifications & PurityExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
SpecificityRANKL
ApplicationELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action TypeINHIBITOR
Mechanism of actionINHIBITOR of Tumor necrosis factor ligand superfamily member 11 inhibitor
Product Description

Denosumab is a monoclonal antibody binding to and inhibiting the receptor activator of RANKL (TNFSF11). It can reduce the risk of vertebral, nonvertebral and hip fractures, also has anti-cancer activity.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Product Properties

Antibody TypePrimary antibody
ClonalityRecombinant
IsotypeHuman IgG1
Light Chain Typekappa
SDS-PAGE26.6 kDa (Light Chain) & 51.2 kDa (Heavy Chain), under reducing conditions; 178.0 kDa, under non-reducing conditions.
Purification MethodProtein A purified
Purity>95%
SourceCHO supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS615258-40-7

Images

Denosumab (anti-TNFSF11) (Ab169305) - SEC
The purity of Denosumab (anti-TNFSF11) (Ab169305) is more than 95% verified by HPLC.

Associated Targets

TNFSF11 Tclin Tumor necrosis factor ligand superfamily member 11 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001443Certificate of AnalysisOct 26, 2023 Ab169305
ZJ23F1001442Certificate of AnalysisOct 26, 2023 Ab169305

Related Documents

References

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3. McClung MR, Lewiecki EM, Cohen SB, Bolognese MA, Woodson GC, Moffett AH, Peacock M, Miller PD, Lederman SN, Chesnut CH et al..  (2006)  Denosumab in postmenopausal women with low bone mineral density..  N Engl J Med,  354  (8): (821-31).  [PMID:16495394]
4. Liu C, Chen X, Zhi X, Weng W, Li Q, Li X, Zou Y, Su J, Hu HG..  (2018)  Structure-based development of an osteoprotegerin-like glycopeptide that blocks RANKL/RANK interactions and reduces ovariectomy-induced bone loss in mice..  Eur J Med Chem,  145  (661-672).  [PMID:29348072]
5. Jiang M, Peng L, Yang K, Wang T, Yan X, Jiang T, Xu J, Qi J, Zhou H, Qian N, Zhou Q, Chen B, Xu X, Deng L, Yang C..  (2019)  Development of Small-Molecules Targeting Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-Receptor Activator of Nuclear Factor-κB (RANK) Protein-Protein Interaction by Structure-Based Virtual Screening and Hit Optimization..  J Med Chem,  62  (11): (5370-5381).  [PMID:31082234]
6. Rinotas V,Papakyriakou A,Violitzi F,Papaneophytou C,Ouzouni MD,Alexiou P,Strongilos A,Couladouros E,Kontopidis G,Eliopoulos E,Douni E.  (2020)  Discovery of Small-Molecule Inhibitors of Receptor Activator of Nuclear Factor-κB Ligand with a Superior Therapeutic Index..  J Med Chem,  63  (20.0): (12043-12059).  [PMID:32955874]
7. Wong, B R BR and 11 more authors..  (1997)  TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells..  The Journal of biological chemistry,    (3):   [PMID:9312132]
8. Anderson, D M DM and 9 more authors..  (1997)  A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function..  Nature,    (13):   [PMID:9367155]
9. Lacey, D L DL and 23 more authors..  (1998)  Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation..  Cell,    (17):   [PMID:9568710]
10. Nagai, M M, Kyakumoto, S S and Sato, N N..  (2000)  Cancer cells responsible for humoral hypercalcemia express mRNA encoding a secreted form of ODF/TRANCE that induces osteoclast formation..  Biochemical and biophysical research communications,    (16):   [PMID:10708588]
11. Body, Jean-Jacques JJ and 14 more authors..  (2003)  A phase I study of AMGN-0007, a recombinant osteoprotegerin construct, in patients with multiple myeloma or breast carcinoma related bone metastases..  Cancer,    (1):   [PMID:12548591]
12. Melchert, Magda M and List, Alan A..  (2007)  The thalidomide saga..  The international journal of biochemistry & cell biology,      [PMID:17369076]
13. Sobacchi, Cristina C and 20 more authors..  (2007)  Osteoclast-poor human osteopetrosis due to mutations in the gene encoding RANKL..  Nature genetics,      [PMID:17632511]
14. Lipton, Allan A and Jun, Susie S..  (2008)  RANKL inhibition in the treatment of bone metastases..  Current opinion in supportive and palliative care,      [PMID:18685421]
15. Akobeng, Anthony K AK and Stokkers, Pieter C PC..  (2009)  Thalidomide and thalidomide analogues for maintenance of remission in Crohn's disease..  The Cochrane database of systematic reviews,    (15):   [PMID:19370685]
16. Reuter, Simone S, Gupta, Subash C SC, Phromnoi, Kanokkarn K and Aggarwal, Bharat B BB..  (2012)  Thiocolchicoside suppresses osteoclastogenesis induced by RANKL and cancer cells through inhibition of inflammatory pathways: a new use for an old drug..  British journal of pharmacology,      [PMID:21955206]
17. Luan, Xudong X and 8 more authors..  (2012)  Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:22664871]

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