Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I
Specifications & Purity
≥2,000 Kunitz units/ml
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Grade
EnzymoPure™
Product Description
BbpDNase I is not base nor sequence specific; however, it does not cleave randomly. It shows preference for cleavage at the 5’ side of pyrimidines, and is particularly pronounced in alternative copolymers (Bernardi et al. 1975, and Lomonossoff et al. 1981). It has been shown that variations in the twist angle are recognized by DNase I (Dickerson and Drew 1981). The specificity of DNase I also depends on the divalent cations present. In the presence of Ca2+ and Mg2+, it causes single strand breaks, and in the presence of Mn2+ double strand breaks have been reported (Junowicz and Spencer 1973, and Campbell and Jackson 1980).
Protein Accession Number: P00639 CATH Classification (v. 3.2.0): Class: Alpha Beta Architecture: 4-Layer Sandwich Topology: Deoxyribonuclease I; Chain A Molecular Weight: 29.1 kDa (Theoretical) Optimal pH: 7.8 Isoelectric Point: 5.08 (Theoretical) Extinction Coefficient: 36,750 cm-1 M-1 E1%,280 = 11.1 Active Site Residues: Tyrosine (Y87) Glutamate (E100) Histidine (H156) Activators: Bivalent metal ions (Junowicz and Spencer 1973b, Poulos and Price 1972, Price 1969, and Price 1975) Inhibitors: 2-mercaptoethanol (Laskowski 1971) 2-nitro-5-thiocyanobenzoic acid (Moore 1981) Actin (Mannherz et al. 2008, and Lazarides and Lindberg 1974) Alfatoxin B2a, G2, G2a, and M1 (non-competitive) (Schabort 1970) EGTA (Moore 1981) and EDTA (Junowicz and Spencer 1973) Sodium dodecyl sulfate (Liao 1975a) Calf spleen inhibitor protein (Laskowski 1971) Carbodiimide and cholesterol sulfate (Iwamori 2000) Iodoacetate (Moore 1981) Applications: DNA removal in primary cell isolation: decreases viscosity providing better yields DNA removal in bioprocessing applications Removing genomic DNA from RNA preparations prior to RT-PCR in vitro transcription Nick translation DNase footprinting Actin binding UV crosslinking of proteins to nucleic acids Radioactive labeling
E1522102