Deoxyribonuclease I from Pichia pastoris (Recombinant,Solution) - ≥2 units/ul, high purity , CAS No.9003-98-9

Item Number

D128589

• Synonyms: Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I
• Specifications & Purity: EnzymoPure™, ≥2 units/ul
• Biochemical and Physiological Mechanisms: DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: EnzymoPure™

Product Description

description：

Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer.

Storage Buffer : 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.

DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.

application：

Recombinant DNase I is suitable for such applications as:

• Removing genomic DNA from RNA preparations prior to RT-PCR

• Degradation of DNA templates after transcription reactions

• Removing unwanted DNA from samples prior to Northern blotting

• Removing DNA during biopharma and bioprocessing procedures