Diethyl pyrocarbonate - 98%, high purity , CAS No.1609-47-8

Item Number

D105557

• Synonyms: diethyl pyrocarbonate|1609-47-8|Diethyl dicarbonate|DEPC|Diethyl oxydiformate|Baycovin|Diethylpyrocarbonate|Dicarbonic acid, diethyl ester|Ethyl pyrocarbonate|Pyrocarbonic acid diethyl ester|Dicarbonic acid diethyl ester|Diethyl pyrocarbonic acid|Piref|Ox
• Specifications & Purity: ≥98%
• Biochemical and Physiological Mechanisms: Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
• Storage Temp: Store at 2-8°C,Argon charged
• Shipped In: Wet ice
• Note: Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Product Description

Diethyl Pyrocarbonate has been shown to inhibit ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. This compound could be useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Diethyl Pyrocarbonate also inhibits PAF Acetylhydrolase (platelet-activating factor acetyl hydrolase), and has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.
Utilized as an antimicrobial additive, a ribonuclease inhibitor, a histidine residue modifier, and as a reagent for conversion of imines to carbamates. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases