Grouped product items

SKU

Size

Availability

Price

Qty

D113494-5mg

5mg

Available within 8-12 weeks(?)

$41.90

D113494-10mg

10mg

In stock

$64.90

D113494-25mg

25mg

Available within 4-8 weeks(?)

$146.90

D113494-100mg

100mg

Available within 4-8 weeks(?)

$526.90

Basic Description

Synonyms	Hydroethidine \| J-100119 \| AKOS015915966 \| FT-0624954 \| PH011085 \| Hydroethidine;PD-MY 003 \| Enoxacinum (Latin) \| NSC-408120 \| 5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine \| Q3027884 \| BCP15712 \| SCHEMBL79004
Specifications & Purity	≥95%
Storage Temp	Protected from light,Store at -20°C,Argon charged
Shipped In	Ice chest + Ice pads
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Hydroethidine operates effectively as a probe for measurement of reactive oxygen species. The dye enters cells freely and is dehydrogenated to ethidium bromide. The probe has been used extensively with NK cell and as a vital dye for identification of proliferation and hypoxic cells in tumors. Studies have been performed using neutrophils and endothelial cells as well as HL60 cells and macrophages. A major advantage of this probe is its ability to distinguish between superoxide and H2O2. Fluorescence emission occurs at around 600 nm. Redox indicator. Blue fluorescence until oxidized to ethidium

Names and Identifiers

IUPAC Name	5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine
INCHI	InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChi Key	XYJODUBPWNZLML-UHFFFAOYSA-N
Canonical SMILES	CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
Isomeric SMILES	CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
WGK Germany	3
PubChem CID	128682
Molecular Weight	315.41
Beilstein	5107482

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

11 results found

Lot Number	Certificate Type	Date	Item
K2320323	Certificate of Analysis	Oct 17, 2023	D113494
K2320352	Certificate of Analysis	Oct 17, 2023	D113494
G2412054	Certificate of Analysis	Sep 29, 2022	D113494
K2212178	Certificate of Analysis	Sep 29, 2022	D113494
K2212179	Certificate of Analysis	Sep 29, 2022	D113494
K2212180	Certificate of Analysis	Sep 29, 2022	D113494
G2219366	Certificate of Analysis	Jun 14, 2022	D113494
G2219367	Certificate of Analysis	Jun 14, 2022	D113494
G2219368	Certificate of Analysis	Jun 14, 2022	D113494
I2222446	Certificate of Analysis	Jun 14, 2022	D113494
L2320012	Certificate of Analysis	Jun 14, 2022	D113494

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Chemical and Physical Properties

Sensitivity	Air sensitive.Light sensitive.

Safety and Hazards(GHS)

WGK Germany	3

Alternative Products

Citations of This Product

1. Tongsheng Huang, Xin Hu, Shiyu Huang, Dong Liu, Lihua Chen, Qinghua Wu, Weifeng Zhu, Xiaoyong Zhang, Meiying Liu, Yen Wei. (2024) One-step synthesis of cyclodextrin-based fluorescent hyperbranched polymer as type I photosensitizer for effective photodynamic therapy. DYES AND PIGMENTS, 223 (111928). [PMID:]
2. Lei Qian, Hu Xu, Ruqiang Yuan, Weijing Yun, Yufang Ma. (2024) Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction. BIOMEDICINE & PHARMACOTHERAPY, 170 (116000). [PMID:38070245]
3. Ping Ping, Shasha Guan, Chaoxue Ning, Ting Yang, Yali Zhao, Pei Zhang, Zhitao Gao, Shihui Fu. (2023) Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction. Nanomedicine-Nanotechnology Biology and Medicine, 54 (16): (102708). [PMID:37788793]
4. Mengmeng Pan, Ming Wang, Linjiao Yang, Yongli Song, Ming Jiang, Xu Yu, Li Xu. (2023) Construction of Metal Organic Framework-Derived Fe-N-C Oxidase Nanozyme for Rapid and Sensitive Detection of Alkaline Phosphatase. Nanomaterials, 13 (18): (2496). [PMID:37764526]
5. Ke Xue, Yongfei Zhao, Saidong Sun, Yuanhang Li, Jiankang Liang, Zhengjian Qi. (2023) NIR-AIEgens nanospheres featuring high-fidelity dynamic lipid droplet targeting, expediting ferroptosis to annihilating tumor in hypoxia. CHEMICAL ENGINEERING JOURNAL, 470 (144125). [PMID:]
6. Zhen Li, Yuanji Feng, Sijia Zhang, Tong Li, Huixin Li, Dianwei Wang, Kai Hao, Chaoliang He, Huayu Tian, Xuesi Chen. (2023) A Multifunctional Nanoparticle Mitigating Cytokine Storm by Scavenging Multiple Inflammatory Mediators of Sepsis. ACS Nano, 17 (9): (8551–8563). [PMID:37129445]
7. Yi Zhao, Yang Liu, Yuying Wang, Shulan Li, Yi Liu, Zhong Lin Wang, Peng Jiang. (2023) The process of free radical generation in contact electrification at solid-liquid interface. Nano Energy, 112 (108464). [PMID:]
8. Yizhong Shen, Xiang Gao, Huanhuan Chen, Yunlong Wei, Hui Yang, Ying Gu. (2023) Ultrathin C3N4 nanosheets-based oxidase-like 2D fluorescence nanozyme for dual-mode detection of organophosphorus pesticides. JOURNAL OF HAZARDOUS MATERIALS, 451 (131171). [PMID:36913745]
9. Xiaoyi Xie, Xiaofang Chen, Yaohui Wang, Maosen Zhang, Yuxiu Fan, Xiupei Yang. (2023) High-loading Cu single-atom nanozymes supported by carbon nitride with peroxidase-like activity for the colorimetric detection of tannic acid. TALANTA, 257 (124387). [PMID:36841014]
10. Na An, Yan Gao, Ying Shi, Xingxing Du, Jianing Cheng, Shuanglong Tang, Pengfei Liu, Huike Yang, Chongshen Guo. (2023) Nickel phosphide for MRI-guided synergistic photo-immunotherapy. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY, 151 (150). [PMID:]
11. Ji Ma, Yang Deng, Tingting Yang, Maoru Li, Jing Shang. (2023) Esculetin Alleviates Nonalcoholic Fatty Liver Disease on High-Cholesterol-Diet-Induced Larval Zebrafish and FFA-Induced BRL-3A Hepatocyte. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2): (1593). [PMID:36675107]
12. Qingqing Liu, Chenchen Xu, Su Chu, Shuai Li, Fengxiang Wang, Yanmei Si, Guojiang Mao, Choufei Wu, Hua Wang. (2022) Covalent organic framework-loaded silver nanoparticles as robust mimetic oxidase for highly sensitive and selective colorimetric detection of mercury in blood. Journal of Materials Chemistry B, 10 (48): (10075-10082). [PMID:36458484]
13. Pinghua Ling, Pei Yang, Xianping Gao, Xinyu Sun, Feng Gao. (2022) ROS generation strategy based on biomimetic nanosheets by self-assembly of nanozymes. Journal of Materials Chemistry B, 10 (46): (9607-9612). [PMID:36112113]
14. Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su. (2022) Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response. Nano Today, 46 (101573). [PMID:]
15. Chenghu Dai, Hao Jia, Wubin Wu, Baipeng Yin, Hong Wang, Ling Wang, Yeteng Zhong, Zihua Wang, Chuang Zhang, Jiannian Yao. (2022) Optically Triggering and Monitoring Single-Cell-Level Metabolism Using Ormosil-Decorated Ultrathin Fibers. LANGMUIR, 38 (32): (9844–9852). [PMID:35926220]
16. Hengrui Zhang, Wenya Jiang, Yaou Peng, Jie Yang, Xiaoying Chu, Ziyue Long, Renlong Li, Qiuwei Liang, Hao Suo, Shuting Wang, Mei Yang, Ji Qi, Dan Ding, Ying-Wei Yang, Bailiang Wang. (2022) Killing three birds with one stone: Near-infrared light triggered nitric oxide release for enhanced photodynamic and anti-inflammatory therapy in refractory keratitis. BIOMATERIALS, 286 (121577). [PMID:35617782]
17. Wei Lu, Shuhan Chen, Haifeng Zhang, Jiajun Qiu, Xuanyong Liu. (2022) FeNC single atom nanozymes with dual enzyme-mimicking activities for colorimetric detection of hydrogen peroxide and glutathione. Journal of Materiomics, 8 (1251). [PMID:]
18. Xiaoyu Huang, Wei Zhang, Yaowei Peng, Lu Gao, Fu Wang, Lan Wang, Xunbin Wei. (2022) A Multifunctional Layered Nickel Silicate Nanogenerator of Synchronous Oxygen Self-supply and Superoxide Radical Generation for Hypoxic Tumor Therapy. ACS Nano, 16 (1): (974–983). [PMID:34962763]
19. Huang Rui, Zhao Zizuo, Jiang Xujie, Li Weiwei, Zhang Lidan, Wang Bin, Tie Hongtao. (2021) Liposomal chrysin attenuates hepatic ischaemia-reperfusion injury: possible mechanism via inhibiting NLRP3 inflammasome. JOURNAL OF PHARMACY AND PHARMACOLOGY, 74 (2): (216-226). [PMID:34791354]

References

1. Upadhyaya G et al.. (2021) A rice R2R3-MYB (OsC1) transcriptional regulator improves oxidative stress tolerance by modulating anthocyanin biosynthesis.. Physiol Plant, 173 (4): (2334-2349). [PMID:34625959]
2. Yang Y et al.. (2022) Ciprofol attenuates the isoproterenol-induced oxidative damage, inflammatory response and cardiomyocyte apoptosis.. Front Pharmacol, 13 (1037151). [PMID:36483733]
3. Halder T et al.. (2018) Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants.. Front Plant Sci, 9 (136). [PMID:29491874]
4. Muto J et al.. (2023) Highly concentrated trehalose induces prohealing senescence-like state in fibroblasts via CDKN1A/p21.. Commun Biol, 6 (13). [PMID:36609486]
5. Zeng X et al.. (2020) Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.. Neurotox Res, 38 (3): (785-792). [PMID:32651843]
6. Tongsheng Huang, Xin Hu, Shiyu Huang, Dong Liu, Lihua Chen, Qinghua Wu, Weifeng Zhu, Xiaoyong Zhang, Meiying Liu, Yen Wei. (2024) One-step synthesis of cyclodextrin-based fluorescent hyperbranched polymer as type I photosensitizer for effective photodynamic therapy. DYES AND PIGMENTS, 223 (111928). [PMID:]
7. Lei Qian, Hu Xu, Ruqiang Yuan, Weijing Yun, Yufang Ma. (2024) Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction. BIOMEDICINE & PHARMACOTHERAPY, 170 (116000). [PMID:38070245]
8. Ping Ping, Shasha Guan, Chaoxue Ning, Ting Yang, Yali Zhao, Pei Zhang, Zhitao Gao, Shihui Fu. (2023) Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction. Nanomedicine-Nanotechnology Biology and Medicine, 54 (16): (102708). [PMID:37788793]
9. Mengmeng Pan, Ming Wang, Linjiao Yang, Yongli Song, Ming Jiang, Xu Yu, Li Xu. (2023) Construction of Metal Organic Framework-Derived Fe-N-C Oxidase Nanozyme for Rapid and Sensitive Detection of Alkaline Phosphatase. Nanomaterials, 13 (18): (2496). [PMID:37764526]
10. Ke Xue, Yongfei Zhao, Saidong Sun, Yuanhang Li, Jiankang Liang, Zhengjian Qi. (2023) NIR-AIEgens nanospheres featuring high-fidelity dynamic lipid droplet targeting, expediting ferroptosis to annihilating tumor in hypoxia. CHEMICAL ENGINEERING JOURNAL, 470 (144125). [PMID:]
11. Zhen Li, Yuanji Feng, Sijia Zhang, Tong Li, Huixin Li, Dianwei Wang, Kai Hao, Chaoliang He, Huayu Tian, Xuesi Chen. (2023) A Multifunctional Nanoparticle Mitigating Cytokine Storm by Scavenging Multiple Inflammatory Mediators of Sepsis. ACS Nano, 17 (9): (8551–8563). [PMID:37129445]
12. Yi Zhao, Yang Liu, Yuying Wang, Shulan Li, Yi Liu, Zhong Lin Wang, Peng Jiang. (2023) The process of free radical generation in contact electrification at solid-liquid interface. Nano Energy, 112 (108464). [PMID:]
13. Yizhong Shen, Xiang Gao, Huanhuan Chen, Yunlong Wei, Hui Yang, Ying Gu. (2023) Ultrathin C3N4 nanosheets-based oxidase-like 2D fluorescence nanozyme for dual-mode detection of organophosphorus pesticides. JOURNAL OF HAZARDOUS MATERIALS, 451 (131171). [PMID:36913745]
14. Xiaoyi Xie, Xiaofang Chen, Yaohui Wang, Maosen Zhang, Yuxiu Fan, Xiupei Yang. (2023) High-loading Cu single-atom nanozymes supported by carbon nitride with peroxidase-like activity for the colorimetric detection of tannic acid. TALANTA, 257 (124387). [PMID:36841014]
15. Na An, Yan Gao, Ying Shi, Xingxing Du, Jianing Cheng, Shuanglong Tang, Pengfei Liu, Huike Yang, Chongshen Guo. (2023) Nickel phosphide for MRI-guided synergistic photo-immunotherapy. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY, 151 (150). [PMID:]
16. Ji Ma, Yang Deng, Tingting Yang, Maoru Li, Jing Shang. (2023) Esculetin Alleviates Nonalcoholic Fatty Liver Disease on High-Cholesterol-Diet-Induced Larval Zebrafish and FFA-Induced BRL-3A Hepatocyte. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2): (1593). [PMID:36675107]
17. Qingqing Liu, Chenchen Xu, Su Chu, Shuai Li, Fengxiang Wang, Yanmei Si, Guojiang Mao, Choufei Wu, Hua Wang. (2022) Covalent organic framework-loaded silver nanoparticles as robust mimetic oxidase for highly sensitive and selective colorimetric detection of mercury in blood. Journal of Materials Chemistry B, 10 (48): (10075-10082). [PMID:36458484]
18. Pinghua Ling, Pei Yang, Xianping Gao, Xinyu Sun, Feng Gao. (2022) ROS generation strategy based on biomimetic nanosheets by self-assembly of nanozymes. Journal of Materials Chemistry B, 10 (46): (9607-9612). [PMID:36112113]
19. Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su. (2022) Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response. Nano Today, 46 (101573). [PMID:]
20. Chenghu Dai, Hao Jia, Wubin Wu, Baipeng Yin, Hong Wang, Ling Wang, Yeteng Zhong, Zihua Wang, Chuang Zhang, Jiannian Yao. (2022) Optically Triggering and Monitoring Single-Cell-Level Metabolism Using Ormosil-Decorated Ultrathin Fibers. LANGMUIR, 38 (32): (9844–9852). [PMID:35926220]
21. Hengrui Zhang, Wenya Jiang, Yaou Peng, Jie Yang, Xiaoying Chu, Ziyue Long, Renlong Li, Qiuwei Liang, Hao Suo, Shuting Wang, Mei Yang, Ji Qi, Dan Ding, Ying-Wei Yang, Bailiang Wang. (2022) Killing three birds with one stone: Near-infrared light triggered nitric oxide release for enhanced photodynamic and anti-inflammatory therapy in refractory keratitis. BIOMATERIALS, 286 (121577). [PMID:35617782]
22. Wei Lu, Shuhan Chen, Haifeng Zhang, Jiajun Qiu, Xuanyong Liu. (2022) FeNC single atom nanozymes with dual enzyme-mimicking activities for colorimetric detection of hydrogen peroxide and glutathione. Journal of Materiomics, 8 (1251). [PMID:]
23. Xiaoyu Huang, Wei Zhang, Yaowei Peng, Lu Gao, Fu Wang, Lan Wang, Xunbin Wei. (2022) A Multifunctional Layered Nickel Silicate Nanogenerator of Synchronous Oxygen Self-supply and Superoxide Radical Generation for Hypoxic Tumor Therapy. ACS Nano, 16 (1): (974–983). [PMID:34962763]
24. Huang Rui, Zhao Zizuo, Jiang Xujie, Li Weiwei, Zhang Lidan, Wang Bin, Tie Hongtao. (2021) Liposomal chrysin attenuates hepatic ischaemia-reperfusion injury: possible mechanism via inhibiting NLRP3 inflammasome. JOURNAL OF PHARMACY AND PHARMACOLOGY, 74 (2): (216-226). [PMID:34791354]

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Dihydroethidium - 95%, high purity , CAS No.104821-25-2