DL-Dithiothreitol solution

Item Number
D755755
Grouped product items
SKUSizeAvailabilityPrice Qty
D755755-10ml
10ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$38.90
D755755-50ml
50ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$113.90
D755755-250ml
250ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$453.90

Basic Description

Specifications & PurityBioUltra, for molecular biology, ~1 M in H2O
Biochemical and Physiological MechanismsDL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine
Storage TempStore at 2-8°C
Shipped InWet ice
GradeBioUltra, for molecular biology
Product Description

Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Application:
DL-dithiothreitol solution has been used:

  • in the treatment of intestinal explants
  • in homogenate preparation of gill cells for enzymatic assays
  • in protein electrophoresis for western blotting
  • in transglutaminase activity
  • in sample buffer for SDS-PAGE
  • in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
  • as a component of protein extraction buffer for cell lysis

Names and Identifiers

PH2.7-5.0
IUPAC Name (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
INCHI InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChi Key VHJLVAABSRFDPM-QWWZWVQMSA-N
Canonical SMILES C(C(C(CS)O)O)S
Isomeric SMILES C([C@H]([C@@H](CS)O)O)S
WGK Germany 3
RTECS EK1610000
Alternate CAS 27565-41-9
PubChem CID 446094
Molecular Weight 154.25
Beilstein 1719760
Reaxy-Rn 1719757

Certificates

Certificate of Analysis(COA)

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Chemical and Physical Properties

Flash Point(°F)235.4 °F
Flash Point(°C)113 °C
Boil Point(°C)125~130°C
Melt Point(°C)42-43°C

Safety and Hazards(GHS)

Pictogram(s) GHS05,   GHS07
Signal Danger
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

H302:Harmful if swallowed

H318:Causes serious eye damage

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P330:Rinse mouth.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P301+P317:IF SWALLOWED: Get medical help.

P305+P354+P338:IF IN EYES: Immediately rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3
RTECS EK1610000
Reaxy-Rn 1719757

Related Documents

Solution Calculators