DL10000 DNA Marker

Item Number

D598085

• Product Name: DL10000 DNA Marker
• Specifications & Purity: 0.09 mg/mL

Product Description

Recommended electrophoresis buffer：TAE /TBE

Product introduction：

DL 10000 DNA Marker is composed of 8 linear double-stranded DNA bands, which is suitable for the analysis of DNA bands in agarose gel electrophoresis, and is not recommended for Acrylamide gel electrophoresis. This product is a ready-to-use product, which already contains 1 × Loading Buffer. According to the needs of the experiment, the sample can be directly loaded for electrophoresis. It is easy to use, suitable for the judgment of long fragment DNA, and the electrophoresis image is clear. The product contains two dyes ( cyan dye and yellow dye ), which are separated during electrophoresis to monitor the migration progress. The cyan dye has the same mobility as the 3-5 kb DNA fragment in 1 % agarose gel, and the yellow dye migrates faster than the primer in 1 % agarose gel ( < 50 bp ).

Ribbon composition：

1000 bp，2000 bp，3000 bp，4000 bp，5000 bp，6000 bp，8000 bp，10000 bp。

 

Matters needing attention：

1.Please instantaneously centrifuge the product to the bottom of the tube before use, and then carry out subsequent experiments ;
2.Please replace the electrophoresis buffer in time and use the new gel to avoid affecting the electrophoresis results ;
3.Recommended electrophoresis conditions for 1 % agarose gel, voltage 4-10V / cm ;
4.Dyeing by GelstainRedTM or other dyes to observe the electrophoretic bands ;
5.Avoid repeated freezing and thawing.
Instruction：
1.Electrophoresis can be carried out with GelstainRed, and 5μL is added to the sample hole of agarose gel for electrophoresis.
2.It is recommended that the electrophoresis conditions are 2-3 % agarose gel, voltage 4-10V / cm.
3.Staining with GelstainRed or other dyes to observe the electrophoretic bands.

Scope of application：

DNA ladder