DL15000 DNA Marker

Item Number

B598088

• Product Name: DL15000 DNA Marker
• Specifications & Purity: 0.064mg/mL

Product Description

Recommended electrophoresis buffer：TAE/TBE

Product introduction：

The 15000bp DNA ladder is composed of seven linear double stranded DNA bands, which is suitable for the analysis of DNA bands in agarose gel electrophoresis and is not recommended for acrylamide gel electrophoresis. This product is ready to use and contains 1 × Loading buffer can directly load samples for electrophoresis according to experimental needs, which is convenient to use and has clear electrophoresis images. The product contains two dyes (cyan dye and yellow dye), which will be separated during electrophoresis to monitor the migration progress. The cyan dye has the same mobility as the 3-5kb DNA fragment in 1% agarose gel, and the yellow dye migrates faster (<50bp) than the primer in 1% agarose gel.

Ribbon composition：

250 bp，1000 bp，2500 bp， 5000 bp，7500 bp，10000 bp，15000 bp。

 

Product characteristic：

It is suitable for accurate determination of DNA fragment size in the range of 250 bp-15000 bp.

Matters needing attention：

1.Freezing and mixing thoroughly when using. 

2.Please replace the electrophoresis buffer in time and use the new gel to avoid affecting the electrophoresis results. 

3.Avoid repeated freezing and thawing.

Instruction： 

1.If Super GelRed is used for electrophoresis, 1-2μL ( 5μL is recommended if combined with common nucleic acid dyes in the market ) is added to agarose gel. In the sample hole, if the sample hole is wider, the sample volume can be appropriately increased for electrophoresis. 

2.It is suggested that the electrophoresis condition is 1 % agarose gel, the voltage is about 5V / cm, and the distance between the positive and negative poles of the electrophoresis tank. 

3.Observe the electrophoretic bands by staining with Super GelRed or other dyes.

Scope of application：

DNA ladder