DNA/RNA Denaturing Loading Buffer

Item Number

D745356

• Specifications & Purity: 2X
• Stability And Storage: Store at -20℃ for up to two years, or at 4℃ for at least one month.
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Aladdin's DNA/RNA Denaturing Loading Buffer (2X) is a 2x concentrated buffer for gel loading of DNA/RNA samples. After dilution to 1X, the buffer still has a high specific gravity and easily sinks into wells, and is clearly visible in blue.This product can be used for routine electrophoresis of double-stranded DNA and total RNA, as well as for electrophoresis of single-stranded DNA, DNA primer, small RNA, or specific RNA purified.This product is suitable for use with agarose /PAGE gels for denaturing DNA/RNA electrophoresis.Nucleic acid dye such as Aladdin's NA-Red (2000X) can be added in samples or gels before electrophoresis, or used to stain gels after electrophoresis.This product can be used in conjunction with the Biotin Northern Blot Kit or the Biotin Northern Blot Kit (for Small RNA) .This product is mainly composed of glycerol, bromophenol blue, and xylene cyanol.

Precautions：

Avoid RNase contamination during operation. Wear a disposable mask when using this product and minimize its exposure to air. This product should be immediately sealed after each use and repeated freeze-thaw should be avoided as much as possible. We also recommend to aliquot this product appropriately when using it for the first time.The formamide in this product is toxic, so please take protective measures when using it.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Mix the DNA or RNA sample with an equal volume of the DNA/RNA Denaturing Loading Buffer (2X), and mix well gently.2.For RNA samples, incubate the mix at 70°C for 5-10 minutes or at 95°C for 1 minute to disrupt the RNA secondary structures, then immediately place on ice. For DNA samples, incubate at 95°C for 5 minutes to fully denature the DNA, then immediately place on ice.3.Load and run the gel as usual.Related Products：Cat. No.Product NamePack SizeD3106Biotin 3'End DNA Labeling Kit20TD3118Biotin Random Prime DNA Labeling Kit10TR0206-100μlSmall RNA Ladder (10-50nt, 9 bands)100μlR0211DNA/RNA Native Loading Buffer (10X)2/10mlR0212DNA/RNA Loading Buffer (2X, for Denaturing PAGE)2/10mlR0215-1ml2X RNA Loading Buffer1mlR0216DNA/RNA Denaturing Loading Buffer (2X)2/10mlR0219Biotin Northern Blot Kit1000cm2R0220Biotin Northern Blot Kit (for Small RNA)1000cm2R0221-500mlMOPS Gel Running Buffer (10X, DNase/RNase free)500mlR0223-500mlTBE (5X, DNase/RNase free)500mlR0225TE (Tris-EDTA Buffer, DNase/RNase free)100/500mlR0227-500mlSSC (20X, DNase/RNase free)500mlR0229-500ml Quick Hybridization Solution500mlR0251-500mlLow Stringency Wash Solution (DNase/RNase free)500mlR0253-500mlHigh Stringency Wash Solution (DNase/RNase free)500mlR0262-250mlNorthern Blot Stripping Solution (Mild)250mlR0266-250mlNorthern Blot Stripping Solution (Moderate)250mlR0268-250mlNorthern Blot Stripping Solution (Harsh)250mlA0303HRP-labeled Streptavidin0.2mlP0018F Ultrapure Water (DNase/RNase-Free, Sterile)100/500mlR0021DEPC-water (DNase、RNase free)100mlR0022DEPC-water (DNase、RNase free)500mlST004LAgarose50gST004B40% Acr-Bis (19:1, Nuclease free)100mlR0123RNase and DNase Away250mlR0125RNase, DNase and DNA Away250mlR0127RNase, DNase, DNA and RNA Away250ml