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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) - Secondary antibody, specific to Rabbit IgG , IgG

    Application:
  • Flow Cytometry
  • IF/ICC
Features and benefitsView More
  • Host species: Donkey
  • Species reactivity(Reacts with): Rabbit
  • Isotype: Donkey IgG
  • Conjugation: Alexa Fluor® 647
Item Number
Ab136882
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab136882-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$69.90
Ab136882-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$447.90
Ab136882-500μg
500μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$237.90

pAb; Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647); Flow, IF, ICC

Basic Description

Product NameDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) - Secondary antibody, specific to Rabbit IgG , IgG
Specifications & PurityExactAb™, Validated, Azide Free, Ex:650nm, Em:668nm, 2.0mg/ml
Host speciesDonkey
ImmunogenNative rabbit IgG
Positive ControlIF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain
ConjugationAlexa Fluor® 647
GradeAzide Free, Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647), Secondary antibody, could be used for Flow, IF, ICC and so on.
Applications
Flow:1/100-1/1000
IF/ICC:1/100-1/1000
Purity
Protein G purified

Product Properties

IsotypeIgG
Purification MethodProtein G purified
FormLiquid
Concentration2.0mg/ml
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles

Images

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) 1/1000. Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.

Application

ApplicationDilution info
Flow Cytometry 1/100-1/1000

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1/100-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800765Certificate of AnalysisAug 03, 2023 Ab136882
ZJ23F0800766Certificate of AnalysisAug 03, 2023 Ab136882

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FAQs and Articles

FAQ
Antibody structure and isotypes
Flow Cytometry
Flow cytometry analysis
How to choose and use antibodies-primary antibody?
How to find the right secondary antibody?
Flow cytometry protocol
Immunocytochemistry (ICC) protocol
RNA dot blot protocol
In-cell ELISA protocol
Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies
Immunofluorescence Staining Protocol (Methanol permeabilization)
Guidelines of Secondary Antibody for Western Blotting

Solution Calculators