Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647), Secondary antibody, could be used for Flow, IF, ICC and so on. Applications Flow:1/100-1/1000 IF/ICC:1/100-1/1000 Purity Protein G purified
Product Properties
Isotype
IgG
Purification Method
Protein G purified
Form
Liquid
Concentration
2.0mg/ml
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles
Images
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) - IF IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) 1/1000. Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) - IHC Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.
Application
Application
Dilution info
Flow Cytometry
1/100-1/1000
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1/100-1/1000
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