DuFinder nucleic acid dye - 10000X in DMSO, high purity

Item Number

D266366

• Specifications & Purity: 10000X in DMSO
• Storage Temp: Store at 2-8°C,Protected from light,Desiccated
• Shipped In: Wet ice

Product Description

This product is dissolved in DMSO. Because the melting point of DMSO is 18.5℃, please put it to room temperature to dissolve fully before use.

DuFinder product introduction

Dufinder is a new type of nucleic acid dye with outstanding advantages such as safety and sensitivity. It can replace ethidium bromide (EB) as a dye for various nucleic acid electrophoresis. Different from EB, which is highly carcinogenic, Dufinder is a kind of dye that has very low toxicity and is safe to use. It can effectively protect experiment operators and the environment.

The fluorescence signal of Dufinder combined with dsDNA can be enhanced by 800-1000 times, and the sensitivity of detecting nucleic acid is about 10 times higher than that of EB staining method on average.

The Dufinder dye has a maximum absorption peak of 470nm, and the nucleic acid bound to the dye exhibits green fluorescence. Observe with a visible light gel transmissometer. UV gel transmissometer can also be used for observation, but the effect is slightly worse. To

DuFinder product parameters

Ex(nm) 508      Em(nm) 533      Solvent: DMSO

DuFinder product features

1. Safety: Dufinder dyes are Huaqing dyes, and their mutagenicity is much lower than EB several times or even dozens of times.

2. Sensitivity: The sensitivity of nucleic acid detection is about 10 times higher than EB staining method on average.

3. Economy: The price is 5-10 times lower than that of imported similar products.

4. Simple operation: There are many ways to use this dye, and the operation is simple.

5. Versatility: Suitable for a variety of nucleic acid electrophoresis analysis. It can stain DNA, RNA, and oligonucleotides.

6. Compatibility: It has no inhibitory effect on commonly used enzymes (such as Taq enzyme, reverse transcriptase, endonuclease, ligase, etc.).

How to use DuFinder

Method 1: Paste dyeing method (the usage is the same as EB) (recommended method)

(1) Add Dufinder nucleic acid dye when making gel. (For example: add 5μL Dufinder 10,000× stock solution to every 50mL agarose solution, and analogy with this ratio).

(2) Perform electrophoresis in accordance with conventional methods.

This method uses relatively less dyes. 500 μL of dye can make about 100 pieces of 50mL glue.

Method 2: Bubble dyeing method

(1) Perform electrophoresis in accordance with conventional methods.

(2) Dilute the Dufinder dye concentrate at a ratio of 10000:1 (or 10000:2) with a buffer solution of pH 7.0～8.5 (such as TAE, TBE or TE) and mix it to make a dyeing solution.

(3) Pour the dye solution into a suitable polypropylene container, put the gel, and cover the container with aluminum foil to protect the dye from light. Shake and stain at room temperature for 10 to 45 minutes. The staining time depends on the gel concentration and thickness. The thicker the gel and the higher the concentration, the longer the dyeing time required. It can also be dyed directly on the polyacrylamide gel (the glass plate must be treated with a silanization solution in advance to prevent the dye from being adsorbed on the glass surface). Pour the prepared working solution gently on the rubber plate, let the working solution evenly cover the entire rubber plate, and dye for 10-30 minutes.

(4) Observation.

DuFinder notes and other instructions

1. The dye is a flower qing dye, which is not carcinogenic, but it may irritate the skin and eyes. Wear gloves when handling it.

2. After the dye is taken out of the refrigerator, return to room temperature, so that the solution is fully melted, and mixed.

3. In the process of conventional alcohol precipitation of nucleic acids, Dufinder can remove all double-stranded nucleic acids.

4. Dufinder has a certain affinity for glass and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing.

5. Storage: Store at 4°C, valid for at least one year.

6. This product is for scientific research use only.