Earle's Balanced Salt Solution (with Ca2+xa0& Mg2+)

Item Number

E743125

• Stability And Storage: Stored at 4 ℃, effective for six months- Valid for one year at 20 ℃. This product can be stored at room temperature for a short period of time.
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Earle's Balanced Salt Solution (with Ca2+ & Mg2+), abbreviated as EBSS (with Ca2+ & Mg2+), produced by aladdin contains 117.24mM NaCl, 5.33mM KCl, 26.19mM NaHCO3, 1.01mM NaH2PO4, 1.80mM CaCl2, 0.813mM MgSO4, and 5.56mM D-Glucose. It is sterile and can be directly used for autophage induction, cell washing, solution preparation, and cell counting dilutions etc.

Precautions：

Use aseptic techniques while handling this product to avoid microorganism contamination.This product contains Ca2+ and Mg2+. also provides EBSS without Ca2+ and Mg2+ .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear lab coat and disposable gloves during the operation.Autophage Induction with EBSS (with Ca2+ & Mg2+)：1. Grow cells in appropriate vessels.2. To induce cell autophagy, remove the culture medium and wash cells 1-3 times with EBSS (with Ca2+ & Mg2+).3. Add appropriate amount of EBSS (with Ca2+ & Mg2+) and incubate for 10, 20, 40 or 60 minutes to induce autophagy. Note: The duration of autophagy induction varies, which needs to be optimized for different cells.4. Wash cells thoroughly to ensure a significant autophagy. It is recommended to detect autophage with AdPlus-mCherry-GFP-LC3B , Ad-mCherry-GFP-LC3B , LC3B Rabbit Polyclonal Antibody (KO Validated) or LC3B antibody (KO Validated) produced by .

Instructions for Use：

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