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EGFR Mouse mAb - Primary antibody, specific to EGFR, IgG2b

Features and benefits
  • Specifications & Purity: ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
  • Short Overview:

    mAb(C4); Mouse anti Human EGFR Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: IgG2b
Item Number
Ab101163
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab101163-10μl (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab101163-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$754.90
Ab101163-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$169.90
Ab101163-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$239.90
Ab101163-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,699.90

mAb(C4); Mouse anti Human EGFR Antibody; WB, IHC; Unconjugated

View related series
Accession#:P00533 EGFR Gene ID:1956

Basic Description

Product NameEGFR Mouse mAb - Primary antibody, specific to EGFR, IgG2b
SynonymsErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody; Cell growth in
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host speciesMouse
SpecificityEGFR
ImmunogenRecombinant EGFR expressed in E.coli (AA 888-1210)
Positive ControlWB: A431, HeLa, MCF7, A549, NIH/3T3 and HUVEC cell lysates. IHC: Human kidney, placent, lung cancer, stomach, adrenal gland, liver cancer tissues.
ConjugateUnconjugated
GradeCarrier Free, ExactAb™, High performance, Validated

Product Properties

IsotypeIgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InDry ice
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

EGFR Mouse mAb (Ab101163) - Western Blot
All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 175 kDa
Observed band size: 169 & 69 kDa
Exposure time: 10.4 s

EGFR Mouse mAb (Ab101163) - Western Blot
All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 175 kDa
Observed band size: 169 & 69 kDa
Exposure time: 2.3 s

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Adrenal gland Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Liver cancer Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets

EGFR Tclin Epidermal growth factor receptor 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationNotes
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404291Certificate of AnalysisApr 09, 2024 Ab101163
ZJ23F1001209Certificate of AnalysisOct 10, 2023 Ab101163
ZJ23F1001208Certificate of AnalysisOct 10, 2023 Ab101163

Related Documents

References

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