EmulsifierX-114 - reagent grade, high purity , CAS No.9036-19-5

8 Citations
Item Number
T101474
Grouped product items
SKUSizeAvailabilityPrice Qty
T101474-250ml
250ml
In stock
$26.90
T101474-500ml
500ml
In stock
$48.90
View related series
Surfactant

Basic Description

Specifications & PurityReagent grade
Biochemical and Physiological MechanismsWidely used nonionic surfactant for recovery of membrane fractions under mild, non-denaturing conditions
Storage TempRoom temperature,Argon charged
Shipped InNormal
GradeReagent Grade
Product Description

EmulsifierX-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of EmulsifierX-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of EmulsifierX-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application:

EmulsifierX-114 has been used:
• To remove endotoxin from solutions of protein using phase separation technique
• In the extraction and purification of DNA from endotoxin
• In the biphasic extraction of lipoproteins from bacterial cell wall
• As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Names and Identifiers

Pubchem Sid488203110
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488203110
PubChem CID 162103267
UN Number 3082
Packing Group III
Beilstein 2315025

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

46 results found

Lot NumberCertificate TypeDateItem
K2322318Certificate of AnalysisOct 07, 2023 T101474
K2322301Certificate of AnalysisOct 07, 2023 T101474
K2322300Certificate of AnalysisOct 07, 2023 T101474
C2429027Certificate of AnalysisOct 07, 2023 T101474
J2311634Certificate of AnalysisOct 07, 2023 T101474
J2311594Certificate of AnalysisOct 07, 2023 T101474
J2311564Certificate of AnalysisOct 07, 2023 T101474
J2311563Certificate of AnalysisOct 07, 2023 T101474
G2319802Certificate of AnalysisJul 12, 2023 T101474
G2319797Certificate of AnalysisJul 12, 2023 T101474
G2319790Certificate of AnalysisJul 12, 2023 T101474
G2319785Certificate of AnalysisJul 12, 2023 T101474
G2319804Certificate of AnalysisJul 12, 2023 T101474
I2307058Certificate of AnalysisJul 12, 2023 T101474
E2316271Certificate of AnalysisMay 10, 2023 T101474
E2316272Certificate of AnalysisMay 10, 2023 T101474
E2316273Certificate of AnalysisMay 10, 2023 T101474
E2316286Certificate of AnalysisMay 10, 2023 T101474
D23261025Certificate of AnalysisApr 14, 2023 T101474
D23261027Certificate of AnalysisApr 14, 2023 T101474
D23261034Certificate of AnalysisApr 14, 2023 T101474
D23261022Certificate of AnalysisApr 14, 2023 T101474
A2312248Certificate of AnalysisDec 28, 2022 T101474
A2312247Certificate of AnalysisDec 28, 2022 T101474
A2312249Certificate of AnalysisDec 28, 2022 T101474
A2312250Certificate of AnalysisDec 28, 2022 T101474
L2215938Certificate of AnalysisNov 25, 2022 T101474
L2215877Certificate of AnalysisNov 25, 2022 T101474
L2215875Certificate of AnalysisNov 25, 2022 T101474
K2208619Certificate of AnalysisOct 28, 2022 T101474
K2208598Certificate of AnalysisOct 28, 2022 T101474
K2208573Certificate of AnalysisOct 28, 2022 T101474
K2208597Certificate of AnalysisOct 28, 2022 T101474
H2222164Certificate of AnalysisAug 13, 2022 T101474
H2222165Certificate of AnalysisAug 13, 2022 T101474
H2222166Certificate of AnalysisAug 13, 2022 T101474
H2222167Certificate of AnalysisAug 13, 2022 T101474
C2203284Certificate of AnalysisFeb 23, 2022 T101474
C2203321Certificate of AnalysisFeb 23, 2022 T101474
C2203285Certificate of AnalysisFeb 23, 2022 T101474
C2203286Certificate of AnalysisFeb 23, 2022 T101474
J2220045Certificate of AnalysisFeb 23, 2022 T101474
C2203291Certificate of AnalysisFeb 23, 2022 T101474
H2210058Certificate of AnalysisFeb 23, 2022 T101474
B2209088Certificate of AnalysisFeb 10, 2022 T101474
I2202144Certificate of AnalysisSep 15, 2021 T101474

Show more⌵

Chemical and Physical Properties

Refractive Index1.488
Flash Point(°F)>230 °F

Safety and Hazards(GHS)

Pictogram(s) GHS09,   GHS05,   GHS07
Signal Danger
Hazard Statements

H315:Causes skin irritation

H302:Harmful if swallowed

H318:Causes serious eye damage

H410:Very toxic to aquatic life with long lasting effects

Precautionary Statements

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P273:Avoid release to the environment.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P310:Immediately call a POISON CENTER or doctor/physician.

P302+P352:IF ON SKIN: wash with plenty of water.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P270:Do not eat, drink or smoke when using this product.

P332+P313:IF SKIN irritation occurs: Get medical advice/attention.

P391:Collect spillage.

P330:Rinse mouth.

P301+P312:IF SWALLOWED: call a POISON CENTER/doctor/... IF you feel unwell.

Class 9

Related Documents

Alternative Products

Citations of This Product

1. Jing Guo, Chang-Yong Tong, Jian-Guang Shi, Xin-Jian Li, Xue-Qin Chen.  (2023)  Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis.  EUROPEAN JOURNAL OF HISTOCHEMISTRY,  67  (3): (3631).  [PMID:37491944]
2. Song Xiaolong, Wang Lei, Liu Min, Pan Renyou, Song Jun, Kong Junhong.  (2023)  Atractylenolide II ameliorates myocardial fibrosis and oxidative stress in spontaneous hypertension rats.  TECHNOLOGY AND HEALTH CARE,  Preprint  (Preprint): (1-12).  [PMID:37483026]
3. Jing Xianwu, Liu Youquan, Zhao Wanwei, Pu Junhong.  (2023)  Synthesis and drag reduction properties of a hydrophobically associative polymer containing ultra-long side chains.  BMC Chemistry,  17  (1): (1-9).  [PMID:37277851]
4. Weixia Xu, Shengmin Yang, Linwei Lu, Qianzhu Xu, Sunyi Wu, Jianfen Zhou, Jiashen Lu, Xingyan Fan, Nana Meng, Yuan Ding, Xudong Zheng, Weiyue Lu.  (2023)  Influence of lung cancer model characteristics on tumor targeting behavior of nanodrugs.  JOURNAL OF CONTROLLED RELEASE,  354  (538).  [PMID:36641120]
5. Qiang Wang, Ying-Rong Wang, Wen-Jing Wei, Hai-Lan Lin, Ping-Jian Yang.  (2022)  Study of the particle characteristics and stability of Ag-NPs naturally generated in soil matrix.  NEW JOURNAL OF CHEMISTRY,  46  (47): (22877-22888).  [PMID:]
6. Weimin Lin, Lei Chen, Wenjing Meng, Kai Yang, Shengjuan Wei, Wei Wei, Jie Chen, Lifan Zhang.  (2022)  C/EBPα promotes porcine pre-adipocyte proliferation and differentiation via mediating MSTRG.12568.2/FOXO3 trans-activation for STYX.  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,  1867  (159206).  [PMID:35870701]
7. Xinyue Guo, Weiqi Zhou, Yanxue Guan, Juan Qin, Bailin Zhang, Miaomiao Zhang, Jilin Tang.  (2022)  The protective effect of biomineralized BSA-Mn3O4 nanoparticles on HUVECs investigated by atomic force microscopy.  ANALYST,  147  (10): (2097-2105).  [PMID:35485407]
8. Ning Wan, Ping Kou, Hong-yi Pang, Yuan-hang Chang, Liu Cao, Chang Liu, Chun-jian Zhao, Cheng-bo Gu, Yu-jie Fu.  (2021)  Enzyme pretreatment combined with ultrasonic-microwave-assisted surfactant for simultaneous extraction of essential oil and flavonoids from Baeckea frutescens.  INDUSTRIAL CROPS AND PRODUCTS,  174  (114173).  [PMID:]

References

1. Y Aida,M J Pabst.  (1990-09-14)  Removal of endotoxin from protein solutions by phase separation using Triton X-114..  Journal of immunological methods,  132  ((2)): (191-195).  [PMID:2170533]
2. P J Newman,M A Knipp,R A Kahn.  (1982-07-15)  Extraction and identification of human platelet integral membrane proteins using Triton X-114..  Thrombosis research,  27  ((2)): (221-224).  [PMID:7135356]
3. J S Brusca,J D Radolf.  (1994-01-01)  Isolation of integral membrane proteins by phase partitioning with Triton X-114..  Methods in enzymology,  228  (182-193).  [PMID:8047007]
4. M Schmidt,M Varsányi,R Thieleczek,L M Heilmeyer.  (1993-07-05)  Identification of a new 80 k isoform of phosphatidylinositol 4-phosphate 5-kinase from bovine brain..  FEBS letters,  325  ((3)): (201-204).  [PMID:8391476]
5. A Sánchez-Ferrer,R Bru,F Garcia-Carmona.  (1989-12-01)  Novel procedure for extraction of a latent grape polyphenoloxidase using temperature-induced phase separation in triton x-114..  Plant physiology,  91  ((4)): (1481-1487).  [PMID:16667205]
6. Rohini Chopra-Dewasthaly,Christine Citti,Michelle D Glew,Martina Zimmermann,Renate Rosengarten,Wolfgang Jechlinger.  (2008-02-06)  Phase-locked mutants of Mycoplasma agalactiae: defining the molecular switch of high-frequency Vpma antigenic variation..  Molecular microbiology,  67  ((6)): (1196-1210).  [PMID:18248580]
7. D V Krishna Pantakani,Marta M Czyzewska,Anna Sikorska,Chiranjeevi Bodda,Ashraf U Mannan.  (2012-01-05)  Oligomerization of ZFYVE27 (Protrudin) is necessary to promote neurite extension..  PloS one,  ((12)): (e29584-e29584).  [PMID:22216323]
8. Halil İbrahim Ulusoy,Ramazan Gürkan,Songül Ulusoy.  (2012-01-24)  Cloud point extraction and spectrophotometric determination of mercury species at trace levels in environmental samples..  Talanta,  88  (516-523).  [PMID:22265535]
9. Ebru Ates,Klaus Mittendorf,Hamide Senyuva.  (2012-02-11)  LC/MS method using cloud point extraction for the determination of permitted and illegal food colors in liquid, semiliquid, and solid food matrixes: single-laboratory validation..  Journal of AOAC International,  94  ((6)): (1853-1862).  [PMID:22320093]
10. Rui Ma,Jun Zhao,Hui-Cong Du,Shuang Tian,Li-Wen Li.  (2012-03-01)  Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction..  Analytical biochemistry,  424  ((2)): (124-126).  [PMID:22370278]
11. Shikha Gupta,Karuna Shanker,Santosh K Srivastava.  (2012-04-03)  HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfia tetraphylla: a study of organic/green solvent and continuous/pulse sonication..  Journal of pharmaceutical and biomedical analysis,  66  (33-39).  [PMID:22464556]
12. Ana C A M Pajuaba,Deise A O Silva,Karine C Almeida,Jair P Cunha-Junior,Carlos P Pirovani,Luciana R Camillo,José R Mineo.  (2012-04-28)  Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle..  Proteomics,  12  ((6)): (820-831).  [PMID:22539433]
13. Marta Toth,Anjum Sohail,Rafael Fridman.  (2012-06-08)  Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography..  Methods in molecular biology (Clifton, N.J.),  878  (121-135).  [PMID:22674130]
14. Amr Ali Mohamed,Egon Matijević.  (2012-06-19)  Preparation and characterization of uniform particles of flufenamic acid and its calcium and barium salts..  Journal of colloid and interface science,  381  ((1)): (198-201).  [PMID:22703985]
15. Adam J Lowe,Cameron L Bardliving,Carl A Batt.  (2012-06-28)  Methods for chromatographic removal of endotoxin..  Methods in molecular biology (Clifton, N.J.),  899  (265-275).  [PMID:22735959]
16. Dimosthenis L Giokas,Qing Zhu,Qinmin Pan,Alberto Chisvert.  (2012-07-13)  Cloud point-dispersive μ-solid phase extraction of hydrophobic organic compounds onto highly hydrophobic core-shell Fe₂O₃@C magnetic nanoparticles..  Journal of chromatography. A,  1251  (33-39).  [PMID:22784694]
17. Demirhan Citak,Mustafa Tuzen.  (2012-09-14)  Cloud point extraction of copper, lead, cadmium, and iron using 2,6-diamino-4-phenyl-1,3,5-triazine and nonionic surfactant, and their flame atomic absorption spectrometric determination in water and canned food samples..  Journal of AOAC International,  95  ((4)): (1170-1175).  [PMID:22970587]
18. Qi Zhao,Jie Ding,Haiyan Jin,Lan Ding,Nanqi Ren.  (2012-09-15)  A green method using a micellar system for determination of andrographolide and dehydroandrographolide in human plasma..  Journal of chromatographic science,  51  ((4)): (341-348).  [PMID:22977119]
19. Hamta Shoaee,Mina Roshdi,Nasibeh Khanlarzadeh,Asadollah Beiraghi.  (2012-09-18)  Simultaneous preconcentration of copper and mercury in water samples by cloud point extraction and their determination by inductively coupled plasma atomic emission spectrometry..  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,  98  (70-75).  [PMID:22983201]
20. Lingling Zhao,Shuxian Zhong,Keming Fang,Zhaosheng Qian,Jianrong Chen.  (2012-09-22)  Determination of cadmium(II), cobalt(II), nickel(II), lead(II), zinc(II), and copper(II) in water samples using dual-cloud point extraction and inductively coupled plasma emission spectrometry..  Journal of hazardous materials,  239-240  (206-212).  [PMID:22995204]
21. Friedrich P Thinnes,Gerhard Burckhardt.  (2012-09-25)  On a fully closed state of native human type-1 VDAC enriched in Nonidet P40..  Molecular genetics and metabolism,  107  ((3)): (632-633).  [PMID:23000107]
22. Zonghao Li,Gong Yu,Jun Song,Qi Wang,Mousheng Liu,Yaling Yang.  (2012-11-22)  Study on the determination of heavy metals in water samples with ultrasound-assisted dispersive liquid-liquid microextraction prior to FAAS..  Water science and technology : a journal of the International Association on Water Pollution Research,  67  ((2)): (247-253).  [PMID:23168620]
23. S Okonogi,W Chaiyana.  (2012-12-12)  Enhancement of anti-cholinesterase activity of Zingiber cassumunar essential oil using a microemulsion technique..  Drug discoveries & therapeutics,  ((5)): (249-255).  [PMID:23229145]
24. Qingwen Deng,Meihui Chen,Lamei Kong,Xia Zhao,Jie Guo,Xiaodong Wen.  (2012-12-26)  Novel coupling of surfactant assisted emulsification dispersive liquid-liquid microextraction with spectrophotometric determination for ultra trace nickel..  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,  104  (64-69).  [PMID:23266677]
25. Georg Hartmann,Michael Schuster.  (2013-01-15)  Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry..  Analytica chimica acta,  761  (27-33).  [PMID:23312311]
26. John E Bradley,Joy M Chan,James S Hagood.  (2013-01-30)  Effect of the GPI anchor of human Thy-1 on antibody recognition and function..  Laboratory investigation; a journal of technical methods and pathology,  93  ((3)): (365-374).  [PMID:23358110]
27. Jameel A Baig,Tasneem G Kazi,Mohammad Balal Arain,Hassan Imran Afridi,Khalida Perveen Mahar.  (2013-03-05)  Quantification of arsenic in dialysate solution and scalp hair samples of kidney failure patients by cloud point extraction and electrothermal atomic absorption spectroscopy..  Journal of AOAC International,  95  ((6)): (1755-1760).  [PMID:23451395]
28. Mustafa Soylak,Erkan Yilmaz,Mehrorang Ghaedi,Mortaza Montazerozohori,Marjan Sheibani.  (2013-03-05)  Cloud point extraction and flame atomic absorption spectrometry determination of lead (II) in environmental and food samples..  Journal of AOAC International,  95  ((6)): (1797-1802).  [PMID:23451399]
29. Hongbing Yang,Elisabeth Yorke,Gemma Hancock,Genevieve Clutton,Nellia Sande,Brian Angus,Redmond Smyth,Johnson Mak,Lucy Dorrell.  (2013-03-19)  Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection..  Journal of immunological methods,  391  ((1-2)): (174-178).  [PMID:23500782]
30. Chunyapuk Kukusamude,Supalax Srijaranai,Masaru Kato,Joselito P Quirino.  (2014-06-09)  Cloud point sample clean-up and capillary zone electrophoresis with field enhanced sample injection and micelle to solvent stacking for the analysis of herbicides in milk..  Journal of chromatography. A,  1351  (110-114).  [PMID:24908154]
31. Mohammad Eftekhari,Mahmoud Chamsaz,Mohammad Hossein Arbab-Zavar,Ali Eftekhari.  (2014-11-19)  Vortex-assisted surfactant-enhanced emulsification microextraction based on solidification of floating organic drop followed by electrothermal atomic absorption spectrometry for speciation of antimony (ΙΙΙ, V)..  Environmental monitoring and assessment,  187  ((1)): (4129-4129).  [PMID:25404541]
32. Pär Comstedt,Markus Hanner,Wolfgang Schüler,Andreas Meinke,Urban Lundberg.  (2014-11-20)  Design and development of a novel vaccine for protection against Lyme borreliosis..  PloS one,  ((11)): (e113294-e113294).  [PMID:25409015]
33. Wuttikon Rodkvamtook,Zhiwen Zhang,Chien-Chung Chao,Erin Huber,Dharadhida Bodhidatta,Jariyanart Gaywee,John Grieco,Narongrid Sirisopana,Manerat Kityapan,Michael Lewis,Wei-Mei Ching.  (2015-03-25)  Dot-ELISA Rapid Test Using Recombinant 56-kDa Protein Antigens for Serodiagnosis of Scrub Typhus..  The American journal of tropical medicine and hygiene,  92  ((5)): (967-971).  [PMID:25802430]
34. Jaqueline Bianchi,Diogo Cavalcanti Cabral-de-Mello,Maria Aparecida Marin-Morales.  (2015-06-16)  Toxicogenetic effects of low concentrations of the pesticides imidacloprid and sulfentrazone individually and in combination in in vitro tests with HepG2 cells and Salmonella typhimurium..  Ecotoxicology and environmental safety,  120  (174-183).  [PMID:26074310]
35. Trícia Maria Ferreira de Sousa Oliveira,Vanessa Figueredo Pereira,Graziella Ulbricht Benvenga,Maria Fernanda Alves Martin,Julia Cristina Benassi,Diogo Tiago da Silva,Wilma Aparecida Starke-Buzetti.  (2015-07-15)  Conjunctival swab PCR to detect Leishmania spp. in cats..  Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria,  24  ((2)): (220-222).  [PMID:26154963]
36. J G Pryde,J H Phillips.  (1986-01-15)  Fractionation of membrane proteins by temperature-induced phase separation in Triton X-114. Application to subcellular fractions of the adrenal medulla..  The Biochemical journal,  233  ((2)): (525-533).  [PMID:2937402]
37. C Bordier.  (1981-02-25)  Phase separation of integral membrane proteins in Triton X-114 solution..  The Journal of biological chemistry,  256  ((4)): (1604-1607).  [PMID:6257680]
38. Jing Guo, Chang-Yong Tong, Jian-Guang Shi, Xin-Jian Li, Xue-Qin Chen.  (2023)  Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis.  EUROPEAN JOURNAL OF HISTOCHEMISTRY,  67  (3): (3631).  [PMID:37491944]
39. Song Xiaolong, Wang Lei, Liu Min, Pan Renyou, Song Jun, Kong Junhong.  (2023)  Atractylenolide II ameliorates myocardial fibrosis and oxidative stress in spontaneous hypertension rats.  TECHNOLOGY AND HEALTH CARE,  Preprint  (Preprint): (1-12).  [PMID:37483026]
40. Jing Xianwu, Liu Youquan, Zhao Wanwei, Pu Junhong.  (2023)  Synthesis and drag reduction properties of a hydrophobically associative polymer containing ultra-long side chains.  BMC Chemistry,  17  (1): (1-9).  [PMID:37277851]
41. Weixia Xu, Shengmin Yang, Linwei Lu, Qianzhu Xu, Sunyi Wu, Jianfen Zhou, Jiashen Lu, Xingyan Fan, Nana Meng, Yuan Ding, Xudong Zheng, Weiyue Lu.  (2023)  Influence of lung cancer model characteristics on tumor targeting behavior of nanodrugs.  JOURNAL OF CONTROLLED RELEASE,  354  (538).  [PMID:36641120]
42. Qiang Wang, Ying-Rong Wang, Wen-Jing Wei, Hai-Lan Lin, Ping-Jian Yang.  (2022)  Study of the particle characteristics and stability of Ag-NPs naturally generated in soil matrix.  NEW JOURNAL OF CHEMISTRY,  46  (47): (22877-22888).  [PMID:]
43. Weimin Lin, Lei Chen, Wenjing Meng, Kai Yang, Shengjuan Wei, Wei Wei, Jie Chen, Lifan Zhang.  (2022)  C/EBPα promotes porcine pre-adipocyte proliferation and differentiation via mediating MSTRG.12568.2/FOXO3 trans-activation for STYX.  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,  1867  (159206).  [PMID:35870701]
44. Xinyue Guo, Weiqi Zhou, Yanxue Guan, Juan Qin, Bailin Zhang, Miaomiao Zhang, Jilin Tang.  (2022)  The protective effect of biomineralized BSA-Mn3O4 nanoparticles on HUVECs investigated by atomic force microscopy.  ANALYST,  147  (10): (2097-2105).  [PMID:35485407]
45. Ning Wan, Ping Kou, Hong-yi Pang, Yuan-hang Chang, Liu Cao, Chang Liu, Chun-jian Zhao, Cheng-bo Gu, Yu-jie Fu.  (2021)  Enzyme pretreatment combined with ultrasonic-microwave-assisted surfactant for simultaneous extraction of essential oil and flavonoids from Baeckea frutescens.  INDUSTRIAL CROPS AND PRODUCTS,  174  (114173).  [PMID:]

Solution Calculators