Enhanced Streptavidin-HRP - Ready-to-use (RTU), high purity

Item Number

E498330

• Specifications & Purity: Ready-to-use (RTU)
• Storage Temp: Store at 2-8°C,Protected from light
• Shipped In: Wet ice

Product Description

Product Characteristics 

Enhanced Streptavidin-HRP is a HRP-conjugate specific for biotinylated antibodies and is characterized by an enhanced molar ratio of >5 HRP enzymes covalently coupled per streptavidin molecule. Enhanced Streptavidin-HRP conjugate enables at least a 4-fold increase in sensitivity in ELISA compared to conventional conjugate and offers pg/fg sensitivity levels in immunoassays, when only minimal amounts of specific antibodies or antigens are available: 

• Signal amplification 

• Ultrasensitive detection 

• Faster assay time 

• Recommended when only minimal amounts of specific antibody or antigen are available 

• Can be used with any biotin labeled probe 

Composition & Properties 

Specificity : Biotinylated probes 

Content : 1 mg HRP/mL stabilized in TRIS buffer 

Activity : 90 - 110% 

Recommended Dilution : 1:1.000 to 1:10.000 

Tips & Tricks 

• Enhanced Streptavidin-HRP can successfully be diluted and stored in Antibody Enhancer (cat. no. A494276). (Not to be used for dilution of Ab’s directed against rabbit immunoglobulins). 

• Avoid batch-to-batch variations and day-to-day variation by preparing a stock solution in your own dilution. 

• Fine-tuning of the polymer backbone is necessary for Immunoblotting, IHC & DNA probe detection. 

Handling & Storage 

• Store solution at 2-8 ℃.