mAb (CD04/3H2); Mouse anti Human EpCAM; WB, IF/ICC, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab191888-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab191888-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab191888-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab191888-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (CD04/3H2); Mouse anti Human EpCAM; WB, IF/ICC, ELISA; Unconjugated
| Product Name | EpCAM Mouse mAb |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Pla |
| Host species | Mouse |
| Specificity | EpCAM |
| Immunogen | Recombinant protein corresponding to Human EpCAM |
| Positive Control | WB: HT-29 whole cell lysate IF/ICC: HT-29 cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated, 高性能 |
| Product Description | Mouse anti Human EpCAM Antibody, clone CD04/3H2, could be used for WB, IF/ICC, ELISA and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
EpCAM Mouse mAb (Ab191888) - Western Blot
All lanes: EpCAM Mouse Antibody (Ab191888) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 32, 39 kDa
Exposure time: 60.0 s
EpCAM Mouse mAb (Ab191888) - ICC/IF
IF analysis of EpCAM (Green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with EpCAM Mouse mAb (Ab191888) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| WB | 1/1,000 – 1/10,000; Predicted molecular weight: 34.9 KDa |
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