Extracellular Solution, ECS

Item Number

E743390

• Stability And Storage: Stored at 4 ℃, effective for six months- Valid for one year at 20 ℃. This product can be stored at room temperature for a short period of time.
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Aladdin's Extracellular Solution (ECS), also known as Isotonic Extracellular Solution, is often used to wash cells in the cell culture process and to prepare fluorescent probes or dye working solutions for live cell stainings. This extracellular solution is better than PBS, HBSS and other buffers in maintaining cell status, especially within 1-2 hours.This product contains 145mM NaCl and appropriate amounts of KCl, CaCl2, MgCl2 and other buffering reagents, with a pH of 7.4±0.1, and is sterilized by 0.22μm filtration. It is a ready-to-use liquid, without the need of any extra preparations prior to use.This product has minimal stimulation to cells when infiltrating the cells for a long time, and thus can replace PBS or HBSS in cell washing. It can also be used as a buffer to effectively maintain cell activity in vitro, and replace the culture medium as a diluent for fluorescent probes or drugs in a short period of time. Especially in live cell staining experiments, it is effective in washing cells without significant effect on the cell status, and is an ideal choice for live cell experiments where serum or phenol red interferes.Because of its minimal stimulation to cells, its ability to effectively maintain cell activity, and the simplicity of its component, this product can also be used as a perfusate in experiments such as confocal observation experiments for continuous drug stimulation or electrophysiological experiments without special requirements for ionic compositions.

Precautions：

Use this product aseptically to avoid contamination by microorganisms. If necessary, add Penicillin-Streptomycin Solution according to the actual situation before use.Discard this product when turned turbid. Turbidity indicates product contamination by microorganisms.If the low temperature affects the cells, the Extracellular Solution should be prewarmed in a 37℃ water bath prior to use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

None