A Broth (Powder)

Directions per Liter:

Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).


Component g/liter
Ammonium Sulfate 1
Potassium Phosphate Dibasic 10.5
Potassium Phosphate Mono 4.5
Sodium Citrate 0.5
Total: 16.5g/Liter
500g makes 32 liters


Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.