bacterial Neisser stain
Summary
Bacterial Neisser stain can be used for budding staining (1) observation of cells (2) detection and classification of cell morphology.
Operation method
bacterial Neisser stain
Principle
Bud cells have high refractive index, dense outer membrane and low permeability, so it is not easy to color them by ordinary staining method, bud cell staining method is designed according to the characteristics that bud cells are both difficult to color, and once colored, it is difficult to decolorize, all the bud cell staining methods are based on the same principle: use dyes with strong coloring power and heat to promote specimen coloring, and then decolorize the body of the bacterium, while the dye on the bud cells is still retained, and after re-staining, the body of the bacterium and the bud cells take on different colors after re-staining.
Materials and Instruments
Bacterial samples
Melan 95% alcohol Glacial acetic acid Crystalline violet Yellow scolding essence Neisser's stain
Microscope
Move
1、Materials
(1) Dyeing solution A
The first solution: Melamine 1g, 95% alcohol 30ml, glacial acetic acid 50ml, distilled water 100ml.
Second solution: crystal violet 1g, 95% alcohol 10ml, distilled water 300ml.
Mix two parts of the above first liquid with one part of the second liquid.
(2) dye solution B, yellow scolding essence 1 or 2g, hot distilled water 300ml, to be dissolved and filtered.
2, staining method
(1) smear according to the conventional method of fixation, add a few drops of Neisser's stain solution A on the smear, 15 ~ 30 Sec, wash.
(2)Re-stain with Neisser's Stain B for 10~30 sec.
(3) Rapidly wash with water, absorb and examine microscopically.
3、Results
The bacterial body was distinct yellow, and the heterostained particles were deep violet-orchid color.
Caveat
1. The smear should be fixed in the usual way to prevent it from falling off.
2. Adjust the objective lens and eyepiece when using a microscope.
Common Problems
After making the smear, with a mixture of two parts of solution I and one part of solution I, stained for 70 seconds, washed . Stained again with the solution, washed again with water, dried and examined microscopically, the bacterial rest chrysanthemums were orange and the heterochromatic particles were dark blue.
For more product details, please visit Aladdin Scientific website.