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Bradford's Reagent Recipe

Recipe

1.Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol

2.Add 100ml of 85% H3PO4 to the solution from step 1

3.Add the solution from step 2 into 500ml of H2O and mix

4.Filter to remove and precipitates

5.Add an additional 350ml of H2O

6.Store at 4℃.


Caution: Always add acid slowly into water and do not add water into acid.

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