Brain Heart Infusion Broth (Powder)

Directions per Liter:

Dissolve 37g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).


Component g/liter
Brain Heart Infusion (from solids) 17.5
Pancreatic Digest of Gelatin 10
Sodium Chloride 5
Dextrose 2
Disodium Phosphate 2.5
Total: 37g/Liter


Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.