Brilliant Green Agar w/Sulfadiazine (Powder)

Directions per Liter:

Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Agar	20
Lactose	10
Sucrose	10
Pancreatic Digest of Casein	5
Peptic Digest of Animal Tissue	5
Sodium Chloride	5
Yeast Extract	3
Phenol Red	0.08
Sulfadiazine	0.08
Brilliant Green	0.0125
	Total: 58.1725g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.