CLED Agar, Bevis (Powder)
CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sand. In the presence of the indicator dye, bromthymol blue, lactose-fermenting bacteria produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Bevis incorporated an additional dye, acid fuchsin, causing lactose fermenting bacteria to produce bright pink colonies and non-lactose fermenting bacteria to show colorless. This medium supports the growth of urinary pathogens and give good colonial differentiation of these bacteria. Since there are no salts (electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. Various authors have used CLED Agar, Bevis as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.
Directions per Liter:
Dissolve 36.2grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
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Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.