ELISA Coating Solution Recipe

Recipe

1.Dissolve 5.3g of Na2CO3 in 900ml distilled H2O.

2.Dissolve 4.2g of NaHCO3 in the solution from step 1.

3.Dissolve 1g sodium azide in the solution from step 2.

4.pH to 9.6.

5.Adjust volume to 1L with additional distilled H2O.