Eosin Methylene Blue Agar, Levine (Powder)

Directions per Liter:

Dissolve 37.5grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)

Component	g/liter
Pancreatic Digest of Gelatin	10
Lactose	10
Dipotassium Phosphate	2
Methylene Blue	0.065
Eosin Y	0.4
Agar	15
	Total: 37.5g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.