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Indirect immunofluorescence of living cells
Summary
The surface of living cells retains a more complete antigen or receptor, first use specific mouse-derived monoclonal antibody to bind to the corresponding antigen on the surface of the cell, and then use fluorescently labeled secondary antibody to bind to it, and according to the measured fluorescence intensity and the percentage of positivity, the density and distribution of the corresponding antigen can be known.
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