micrography

Summary

It is a kind of technology that uses micrographic device to record the microstructure of the objects observed in the field of view of microscope for further analysis and research. It has become a routine and indispensable research technique in scientific research, especially in the fields of medicine and biology.

Operation method

micrography

Principle

Micrography is a technique of taking an optical image of a sample being examined in the field of view of a microscope by means of a photographic device.

Materials and Instruments

Biological Samples
Developer Fixer
Duplex microscope Monochrome film Developing canister Thermometer Oven Darkroom bag Dark box Photographic paper Scissors Plastic plate Beaker Measuring cylinder Balance

Move

1. Installation and commissioning of micrographic devices.
2. Optical path adjustment of the microscope: alignment of the illumination beam with the optical axis of the microscope's optical system.
3. Load film.
4. Framing and focusing.
5. Exposure: 1/15 s (should be metered first: green light is on for correct exposure).
6. Black and white film processing: develop 3 min → stop display 10 sec → fixing 15 min → washing: 30 min of running water → drying.
7. Enlargement of printing: focusing on the scene → exposure → development → stop displaying 10 sec → fixing 15 min → washing: 30 min of running water rinsing.
8. Varnish shear.
9. Experimental results and analysis: independently complete the photographic operation, submit a photograph and a brief description of the sample photographed; analyze the main problems that should be noted in the shooting and improvement measures.

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