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NP-40 Cell Lysis Buffer

Recipe 

  • 50mM Tris-HCl pH 8.0

  • 150mM NaCl

  • 1% NP-40

  • plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each

  • Add 1mM PMSF immediately before use.

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