Nutrient Agar (Powder)
Nutrient Agar was suggested by the American Public Health Association (APHA) in the early 1900's as a standard culture medium for use in bacterial processing in water analysis. This is recommonded for the bacterial examination of water milk. It now continues to be a widely used general purpose medium for growing nonfastidious organisms. It is specified in many standard methods procedures for examining foods, dairy products, water, and other materials.
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Directions per Liter:
Dissolve 23.0 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.