Plasma D-dimer assay

Summary

Plasma D-dimer assay (D-dizller, D-D) detection is commonly used in the following two methods.

1, latex agglutination method, subject to the plasma added to the latex particles labeled with D-dimer monoclonal antibody suspension if the plasma contains higher than 0.5mg / L of D-dimer, it will be combined with the antibody on the latex particles. At this point the latex particles agglutinate.

2、ELISA method The D-dimer monoclonal antibody is encapsulated in an enzyme-labeled reaction plate and added to the plasma to be examined, the D-dimer (antigen) of the plasma binds to the D-dimer antibody encapsulated in the reaction plate, and then the enzyme-labeled D-dimer antibody is added to bind to the encapsulated D-dimer, and finally the substrate is added. Then enzyme-labeled D-dimer antibody is added to bind to the encapsulated D-dimer, and finally the substrate is added to develop the color, and the shade of the color is positively correlated with the amount of D-dimer in the plasma, and the measured A-value can be used to calculate the amount of D-dimer in the plasma in the standard curve.

This experiment is from the laboratory instruction of Mudanjiang Medical College undergraduate 5-year testing program.

Operation method

Plasma D-dimer assay

Principle

The following two methods are commonly used for plasma D-dimer assay (D-dizller, D-D) testing. (1) latex agglutination method; (2) ELISA method

Move

1. In the latex agglutination method, a suspension of latex particles labeled with D-dimer monoclonal antibody is added to the plasma being tested. If the plasma contains more than 0.5 mg/L of D-dimer, it binds to the antibody on the latex particles. At this point the latex particles agglutinate.

2. ELISA The D-dimer monoclonal antibody is encapsulated in an enzyme-labeled reaction plate and added to the tested plasma, the D-dimer (antigen) of the plasma binds to the D-dimer antibody encapsulated in the reaction plate, and then the enzyme-labeled D-dimer antibody is added to bind to the encapsulated D-dimer. Then enzyme-labeled D-dimer antibody is added to bind to the encapsulated D-dimer, and finally the substrate is added to develop the color, and the shade of the color is positively correlated with the amount of D-dimer in the plasma, and the measured A-value can be used to calculate the amount of D-dimer in the plasma in the standard curve.

Reference value: Negative by latex agglutination: ELISA: less than 400 μg/L.

Common Problems

Clinical significance: secondary fibrinolysis (e.g., DIC) is positive or elevated, while primary fibrinolysis is positive or not elevated, which is an important indicator for differentiating between the two.

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