Pregnancy test

Summary

Pregnant women's urine to be tested (containing human chorionic gonadotropin, HCG) is interacted with known anti-HCG antibodies, thus inhibiting the binding of anti-HCG antibodies to HCG on the surface of the sensitized latex particles, whereupon latex agglutination is inhibited.

Operation method

indirect agglutination inhibition method

Principle

After mixing the sample to be tested with the known antibody for a certain period of time, and then mixing it with the latex particles sensitized by the corresponding antigen, if the sample to be tested contains the corresponding antigen, it can bind to the added antibody. When the sensitized latex particles are added, there is no corresponding antibody binding to the antigen on the surface of the latex particles, and thus no agglutination phenomenon occurs, which is an inhibition reaction of latex agglutination, so that those who do not agglutinate are positive, indicating that the samples to be tested contain the corresponding antigens, and those who agglutinate are negative, suggesting that the samples to be tested do not contain the corresponding antigens.

Materials and Instruments

Urine to be tested HCG-positive urine of pregnant women
Adsorbed HCG polystyrene latex antigen Rabbit anti-human HCG immune serum (antibody) Saline
Slide (or black concave plate) Dropper Toothpick Special pencil Optical microscope

Move

1. Take a slide and place it on the laboratory bench, divide it into three compartments with a special pencil and indicate 1, 2 and 3.

2. Take a drop of saline water and add it to compartment 2, then take a drop of urine of the pregnant woman to be tested and add it to compartment 1, and take a drop of urine of a positive pregnant woman and add it to compartment 3.

3. Add one drop of rabbit anti-human HCG immune serum to each of the three compartments 1, 2 and 3, mix the reaction solution thoroughly with a toothpick, and leave for 30 s to 1 min.

4. Add one drop of HCG-sensitized latex antigen to each of the three compartments 1, 2 and 3, mix well with a toothpick and leave for about l0 min.

Results:

The presence of uniform fine agglutinated particles in the first frame is considered negative (non-pregnant urine); the presence of a homogeneous latex liquid that does not agglutinate is considered positive (pregnant urine).

Caveat

1. The diagnostic reagents used must be used within the validity period and should be shaken well before use.

2. The dropper and the toothpick for stirring the reaction solution should not be shared.

3. the sample should not be too much, the slide should be placed flat, in order to prevent the two compartments of the reaction solution overflow mixed. 4. if the visual observation is not clear enough, the slide can be placed in the eye.

4. If the visual observation is not clear enough, the slide can be placed under a microscope and observed with low magnification.

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