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Red Blood Cell (RBC) Lysis Buffer

Recipe

1.Dissolve the following in 800ml distilled H2O

  • 8.3g NH4Cl

  • 1.0g KHCO3

  • 1.8ml of 5% EDTA

2.Filter sterilize through 0.2um filter

3.qs to 1000ml with distilled H2O

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