Rh blood grouping test
Summary
Master the principles and techniques of enzyme-mediated determination of Rh blood group, this experiment is from the Mudanjiang Medical College undergraduate 5-year clinical laboratory guide.
Operation method
Rh blood grouping test
Principle
Rh blood group identification system uses incomplete antibody serum, belongs to IgG type, small molecular weight. In the saline medium can not occur with the corresponding red blood cells agglutination, papain can destroy the red blood cells in the surface charge of salivary acid. Thus reduce the surface charge of erythrocytes, so that it can be close to the specific incomplete antibody can be treated with the enzyme with the corresponding antigen erythrocyte agglutination.
Materials and Instruments
Anti-D Incomplete Antibody Serum Papain Liquid
Move
1, the preparation of enzyme-treated erythrocyte county liquid: the subject, D control, d control three kinds of erythrocytes washed once with saline, centrifugation, each take the pressure of the erythrocytes 1 drop, respectively, add the above preparation of papain l drop. Mixed at 37 ℃ water bath l0min, remove, with saline and then wash 1 time, each formulated into 5% erythrocyte saline suspension.
2, take out the above three kinds of enzyme treatment of erythrocyte suspension, each 1 drop in the three test tubes have been labeled.
3, respectively, in each test tube with anti-D incomplete antibody serum l drops, mix well, placed in a 37 ℃ water bath and removed gently shaking, observe the results.
4, the results of the experiment to determine: all tubes do not appear agglutination for the "d", D control tube and the subject appeared agglutination, while the d control tube no agglutination for the "D". Due to the "D" antigenic weakness, the subject can be no agglutination. Can not be judged immediately, at this time must be discharged "D" possible.
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