SOB Broth (Powder)

Directions per Liter:

Suspend 30.5g per liter of ddH2O. Mix thoroughly. Adjust to desired pH. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
MgSO4•7H2O	5
Sodium Chloride	0.5
Tryptone	20
Yeast Extract B	5
	Total: 30.5g/Liter
	500g makes 16.4 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.

Homogeneous: Proprietary blending technology ensures complete mixing of components.