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SSPE 20X

Recipe

1.Dissolve the following in 800ml of distilled H2O.

 175.3g of NaCl

 27.6g of NaH2PO4-H2O

 7.4 g of EDTA

2.Adjust the pH to 7.4 with 10N NaOH.

3.Adjust the volume to 1L with additional distilled H2O.

4.Sterilize by autoclaving.

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