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Tissue Homogenization Buffer for ELISA
Recipe
Stock Solution:
1.Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water
2.Mix and aliquot into 70ul single use aliquots
To prepare homogenization buffer:
1.Add 50ul of stock solution to 100ml of PBS
2.Sterilze by filtration
Final concentration is 0.5ug/ml
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