Tissue Homogenization Buffer for ELISA

Recipe

Stock Solution:

1.Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water

2.Mix and aliquot into 70ul single use aliquots

To prepare homogenization buffer:

1.Add 50ul of stock solution to 100ml of PBS

2.Sterilze by filtration

Final concentration is 0.5ug/ml