Tris-Buffered Saline (TBS)

Recipe

1.Dissolve the following in 800ml of distilled H2O.

• 8g of NaCl, 0.2g of KCl

• 3g of Tris base

2.Add 0.015g of phenol red.

3.Adjust the pH to 7.4 with HCl.

4.Add distilled H2O to 1L.

5.Sterilize by autoclaving.