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Tris-Buffered Saline (TBS)

Recipe

1.Dissolve the following in 800ml of distilled H2O.

  • 8g of NaCl, 0.2g of KCl

  • 3g of Tris base

2.Add 0.015g of phenol red.

3.Adjust the pH to 7.4 with HCl.

4.Add distilled H2O to 1L.

5.Sterilize by autoclaving.

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