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Tris-Buffered Saline Tween-20 (TBST)
Recipe
1.Dissolve the following in 800ml of distilled H2O
8.8 g of NaCl
0.2g of KCl
3g of Tris base
2.Add 500ul of Tween-20
3.Adjust the pH to 7.4
4.Add distilled H2O to 1L
5.Sterilize by filtration or autoclaving
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