Exoglycosidase Clean-up Plate
Applications Post-exoglycosidase Clean-up
Applications：For removal of exoglycosidase enzymes and other protein material following glycan
enzymatic digestion/ sequencing. This will prevent contamination of HPLC columns
during subsequent chromatographic analysis. The plate can also be used to remove
exoglycosidases or other proteins before mass spectrometry analysis of glycans.
Number of Samples： Sufficient for up to 96 samples.
Amount of Sample： Up to 350 µL per well
Centrifugal Force： 1500 x g
Operating Vacuum： 10-20 in Hg (approx. 0.35-0.7 bar)
Suitable Samples： Unlabelled and fluorophore labelled (e.g. 2-AB, 2-AA or procainamide labelled) glycans released from glycoproteins or other sources and treated with exoglycosidase enzymes.
Storage： Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the products should be stable for 36 months from date of purchase.
Shipping： The product should be shipped at ambient temperature.
Clean-up Procedure
1，Method
Apply the samples onto the clean-up plate
Place a 96-well collection plate inside the vacuum manifold. Assemble the manifold with the post-exoglycosidase clean-up plate on top ensuring that the collection plate is inline with the wells (if using centrifugation instead, place the clean-up plate directly on top of the collection plate).Ensure that the distance between the collection plate and the manifold top is as small as possible to reduce the gap between the clean-up plate and the collection plate and prevent sample crosscontamination.
Pipette the glycan samples into the post-exoglycosidase clean-up plate wells. Wash out each sample vial with 100 µL of water and add this to the clean-up plate wells. Apply a vacuum and adjust to between -0.3 and -0.5 bar until the liquid has all gone through the wells. Open the tap to release the vacuum.（The maximum pressure used should be no more than 0.7 bar.）
Wash the wells of the clean-up plate
Pipette 100 µL of water into each well to wash through any remaining sample. Apply a vacuum and adjust to between -0.3 and -0.5 bar until the liquid has all gone through the wells. Open the tap to release the vacuum.