Frequently asked Questions about inhibitors and antagonists related products（FAQ）
1. What is the product used for? Who can use it?
All of our products are potentially harmful and are for laboratory research and development only. Cannot be used for pharmaceutical, veterinary, domestic, agricultural, food, cosmetic or any other human purposes. It should only be used by scientific professionals trained in laboratory procedures.
All our products come with a Material safety data sheet which includes advice on safe handling of the product. However, due to the novel characteristics of our product, we do not fully understand its potential hazards. Therefore, please ensure that you always take all relevant safety precautions.
2. What dose of antagonist or signal transduction tool should be used in the body?
The dosage is complex and depends on a number of factors, including application method (intravenous, intramuscular, or intraperitoneal), bioavailability, half-life, liver and intestinal clearance, protein binding, drug interactions, and tissue specific distribution and accumulation. We do not conduct any in vivo biological studies of our products, so we recommend that you review the published literature for reference doses.
3. What dose of antagonist or signal transduction tool should be used in vitro?
The amount of product required depends on a number of factors, including target accessibility, cell permeability, incubation time, cell type or assay method used, preferably by reviewing the literature to determine IC50, EC50 or Ki. In terms of inhibitors, if the published Ki or IC50 value is known, we recommend that you use 5 to 10 times higher than that value to maximize enzyme or receptor activity. If no published values are available, we recommend that you perform a dose-response experiment (running appropriate controls) and utilize Michaelis-Menten dynamics to determine Ki values.
4. How do you determine that a compound has cellular permeability?
There is currently no easy way to predict whether a product has cellular permeability. In general, charged molecules are not cellular permeable. Phosphorylated modified compounds, such as mono-butyl-camp and dibutyl-CAMP, have cellular permeability. High molecular weight peptides do not normally have cellular permeability under normal conditions.
5. What is the purity and quality of the product? How to determine?
The purity of our biochemical reagents is extremely high, usually above 98%. A full range of techniques can be used to determine chemical purity and quality, including HPLC, chiral HPLC, NMR, microanalysis, optical rotation analysis, TLC, and mass spectrometry. For details, see the Product Licenses attached to each product.
6. What if you can't see the product in the bottle?
Products sold in small quantities may not be easy to see because the product may cover the bottom or walls of the bottle. Therefore, when stabilizing the product, please ensure that the solvent is in contact with all parts of the bottle.
7. Do different batches of products have the same appearance?
It is normal for different batches of the same product to differ slightly in appearance and color. However, this does not affect the purity or quality of the lot's proprietary product license, nor does it affect product performance.