User Manual of Ready to use RC Membrane Dialysis Bag

1、Technical parameters of membrane:

Biotech RC membrane (regenerated cellulose), with heavy metal ions and sulfide content of trace level, wet type, immerged in 0.05% sodium azide anti-corrosion storage solution, ready to use type, without pretreatment, and used after washing with distilled water; Biotech RC membrane has extensive chemical compatibility, and can withstand weak acids, weak bases or diluted strong acids, strong bases, and most alcohols; Weak polar organics or diluted strong polar organics, such as DMSO, contact with strong polar organic solvents may damage RC membrane; The standard RC membrane can be used between pH 2-12 and temperature 4-132 ° C.

2、Storage conditions:

The dialysate membrane is immersed in the storage solution and placed in a sealed environment between 4-25 ° C. It is recommended to store it in a sealed refrigerator at 4 ℃.

Usage of dialysis bag:

1. First, wear gloves, and cut the dialysis bag into small sections of appropriate length (about 10cm). The length can be as required, but there must be enough containers to contain it.

2. Thoroughly clean the dialysis bag with distilled water, and gently hold both ends with hands to check whether there is any leakage bag. The dialysis bag preservation solution contains preservatives, which can inhibit active substances. It should be rinsed repeatedly with distilled water (pure water or deionized water) for at least three times, and it is better to soak in distilled water for 30 minutes before use.

3. The unused dialysis membrane shall be put back into the storage solution for sealed storage. Gloves must be worn when contacting the dialysis membrane.

4. During use, one end is clamped with a dialysis bag clamp. After being filled with water, pressure is properly applied with your finger to check for no leakage. The other end is also repeated again to avoid that the clamp is not tight enough. Then fill the sample, usually leaving one third to one half of the space to prevent the bag from bursting due to excessive water and buffer solution entering the bag during dialysis. After the sample is loaded, the bag mouth is clamped with a clamp to conduct dialysis. In order to speed up dialysis, magnetic stirrer can be used in addition to replacing the dialysis buffer for many times. The container for dialysis should be larger, and large beakers, large cylinders and plastic buckets can be used. The dialysis time can be determined according to the sample concentration, or overnight dialysis, and the dialysis time should be 24-48 hours. Hang the dialysis bag with a line to keep the bag in suspension, which can also accelerate the dialysis speed.