ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species
Rabbit
Specificity
FGF5
Immunogen
Recombinant FGF5 expressed in E.coli (AA 23-268)
Positive Control
WB: NIH/3T3, Jurkat and HUVEC cell lysates.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human FGF5 Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 0.5-2µg/mL
Protein Function
Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
FGF5 Antibody (Ab103322) - Western Blot All lanes: FGF5 Antibody (Ab103322) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 32 kDa Exposure time: 8.8 s
1.Haub, O O, Drucker, B B and Goldfarb, M M.. (1990) Expression of the murine fibroblast growth factor 5 gene in the adult central nervous system.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:1700424]
2.Zhan, X X, Bates, B B, Hu, X G XG and Goldfarb, M M.. (1988) The human FGF-5 oncogene encodes a novel protein related to fibroblast growth factors.. Molecular and cellular biology, [PMID:3211147]
3.de Vries, C J CJ, van Achterberg, T A TA, Horrevoets, A J AJ, ten Cate, J W JW and Pannekoek, H H.. (2000) Differential display identification of 40 genes with altered expression in activated human smooth muscle cells. Local expression in atherosclerotic lesions of smags, smooth muscle activation-specific genes.. The Journal of biological chemistry, (4):[PMID:10823842]
4.Hanada , K K, Perry-Lalley, D M DM, Ohnmacht, G A GA, Bettinotti, M P MP and Yang, J C JC.. (2001) Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas.. Cancer research, (15):[PMID:11454700]
5.Higgins, Claire A CA and 7 more authors.. (2014) FGF5 is a crucial regulator of hair length in humans.. Proceedings of the National Academy of Sciences of the United States of America, (22):[PMID:24989505]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.