Fluorescein isothiocyanate isomer I is an amine-reactive reagent for the FITC labeling of proteins, microsequencing of proteins, and peptides (HPLC).Fluorescent marker. Amine reactive fluorescein isomer. Yields conjugates with superior stability compared
Storage Temp
Store at 2-8°C,Argon charged
Shipped In
Wet ice
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
FITC (isomer I) is a derivative of fluorescein. FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. FITC can be easily used as labelling agent of various substrates such as proteins or polysaccharides. Isothiocyanate easily react with nucleophiles such as amines under mild conditions. FITC can also be used in flow cytometry.
Structure
Fluorescein isothiocyanate (widely known as FITC) is a derivative of fluorescein which contains an isothiocyanate group at either position 5 or position 6 of the bottom benzene ring.
Spectral data
FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm.
Application
FITC has a wide-range of applications. Including fluorescent labelling of antibodies and flow cytometry. FITC-functionalized products e.g. FITC-dextrans are very useful for studying permeability and transport in a large variety of cells and tissues e.g. intestinal, neoplastic, and ocular tissues as well in research related to brain and neural system.
1.Kashiyama T, Oda K, Ikeda Y, Shiose Y, Hirota Y, Inaba K, Makii C, Kurikawa R, Miyasaka A, Koso T et al.. (2014) Antitumor activity and induction of TP53-dependent apoptosis toward ovarian clear cell adenocarcinoma by the dual PI3K/mTOR inhibitor DS-7423.. PLoS One, 9 (2):(e87220). [PMID:24504419]
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