Fluorouracil (5-Fluorouracil, 5-FU) - 10mM in DMSO, high purity , CAS No.51-21-8(DMSO)

Item Number
F408985
Grouped product items
SKUSizeAvailabilityPrice Qty
F408985-1ml
1ml
Available within 4-8 weeks(?)
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$392.90

DNA/RNA Synthesis Inhibitors

Basic Description

Specifications & PurityMoligand™, 10mM in DMSO
Biochemical and Physiological MechanismsFluorouracil (5-Fluorouracil, 5-FU, NSC 19893) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells. Fluorouracil induces apoptosis and can be used in the treatment of HIV.
Storage TempStore at -80°C
Shipped InIce chest + Ice pads
GradeMoligand™
Product Description

Information

Fluorouracil (5-Fluorouracil, 5-FU) Fluorouracil (5-Fluorouracil, 5-FU, NSC 19893) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells. Fluorouracil induces apoptosis and can be
In vitro

Adrucil is an analogue of uracil with a fluorine atom at the C-5 position in place of hydrogen. It rapidly enters the cell using the same facilitated transport mechanism as uracil. Adrucil is converted intracellularly to several active metabolites: fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP). The Adrucil metabolite FdUMP binds to the nucleotide-binding site of TS, forming a stable ternary complex with the enzyme and CH2THF, thereby blocking binding of the normal substrate dUMP and inhibiting dTMP synthesis. Metabolite of Adrucil also can be misincorporated into DNA, leading to DNA strand breaks and cell death. The pro-apoptosis effects of Adrucil may be related to its activation of tumor suppressor p53. Loss of p53 function reduces cellular sensitivity to Adrucil. Adrucil is able to inhibit the survival and induce apoptosis of a board range of cancer cells. Adrucil suppresses viabilities of the nasopharyngeal carcinoma cell line CNE2 and HONE1 , pancreatic cancer cell lines Capan-1 , and human colon carcinoma cell line HT-29 with IC50 of 9 μg/mL, 3 μg/mL, 0.22 μM, 2.5 μM, respectively.

In vivo

Adrucil is widely used in the treatment of a range of cancers, including colorectal and breast cancers. 100mg/kg Adrucil significantly suppresses tumor growth of murine colon carcinomas Colon 38 with tumor-doubling time (TD), growth-delay factor (GDF), and T/C of 26.5 days, 4.4, and 14%.
Cell Data

cell lines:

Concentrations:~25 μM

Incubation Time:7 days

Powder Purity:≥99%

Names and Identifiers

Canonical SMILES FC1=CNC(=O)NC1=O
Molecular Weight 130.08

Certificates

Certificate of Analysis(COA)

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Related Documents

Citations of This Product

1. Lu Yang-yang, Zhu Chun-yang, Ding Yi-xin, Wang Bing, Zhao Shu-fen, Lv Jing, Chen Shu-ming, Wang Sha-sha, Wang Yan, Wang Rui, Qiu Wen-sheng, Qi Wei-wei.  (2023)  Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway.  Cell Death Discovery,  (1): (1-15).  [PMID:38086844] [10.1038/s41420-023-01752-z]

References

1. Lu Yang-yang, Zhu Chun-yang, Ding Yi-xin, Wang Bing, Zhao Shu-fen, Lv Jing, Chen Shu-ming, Wang Sha-sha, Wang Yan, Wang Rui, Qiu Wen-sheng, Qi Wei-wei.  (2023)  Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway.  Cell Death Discovery,  (1): (1-15).  [PMID:38086844] [10.1038/s41420-023-01752-z]

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