This reagent is made by heating and refluxing sodium tungstate, sodium molybdate, phosphoric acid, and concentrated hydrochloric acid in a certain proportion, and then refining them with lithium sulfate. It has a golden and transparent color and is a commonly used reagent for determining the content of proteins, peptides, polyphenols, tyrosine, and other substances in laboratory solutions. The acidity of this product is 2N. Please use it directly or dilute it appropriately according to experimental needs. This reagent can determine protein concentrations ranging from 0.05 to 1mg/mL. The linear relationship is best when the protein concentration is within the range of 0.05 to 0.5mg/mL. If the protein concentration of the test sample exceeds this range, please dilute it appropriately to the appropriate concentration range. This reagent is corrosive and should be avoided from contact with skin, mucous membranes, etc; When the color of the reagent turns dark green, it should be discarded. This reagent has a concentration of 2N and can be diluted with distilled water or deionized water according to specific requirements during use. This product is only suitable for scientific research and is prohibited from being used for clinical diagnosis or other purposes. Pay attention to sealed storage to avoid volatile and corrosive substances. Preparation before the experiment: 1. Preparation A1: Weigh 20g anhydrous sodium carbonate and 4g sodium hydroxide and dissolve them in 1L of water; 2. Preparation A2: Weigh 0.2g of copper sulfate and dissolve it in 20mL of water; 3. Preparation A3: Weigh 0.4g of potassium sodium tartrate and dissolve it in 20mL of water; On the day of measurement, mix A1: A2: A3 in a volume ratio of 100:1:1 to obtain reagent A. Mix and let it sit for 30 minutes before use. Mix A2 and A3 first, and then mix the mixture with A1. Reversing the order may result in precipitation. Reagent A can only be used on the same day. If precipitation or turbidity occurs, it needs to be re prepared. The three solutions can be stored separately at room temperature for a long time. 4. First, prepare a 2mg/ml protein mother liquor, and then dilute it into protein standard solutions of various concentrations according to the "Appendix". 5. Dilute the Folin phenol reagent from 2N to 1N for use. Operation steps (total reaction volume 5.6ml):
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Sample number label
Label 0
Label 1
Label 2
Label 3
Label 4
Label 5
Label 6
To be tested
1.Add sample
Add protein standard or test protein sample (mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
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Protein concentration (mg/mL)
0
0.05
0.10
0.20
0.25
0.40
0.50
To be tested
2.Add A
Add A5ml of reagent to each tube in sequence; After mixing, let it stand at room temperature for 10 minutes;
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3.Add B
Add 0.5m of reagent B (Folin phenol reagent) to each tube in sequence (mix immediately for each tube added); Leave at room temperature for 30 minutes;
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4.Measurement
Under 750nm wavelength light, zero the equipment with a standard tube and measure the absorbance (OD value) of each tube;
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5.Calculation
Draw a standard curve based on the data measured by tubes 0 to 6, and calculate the concentration of each sample to be tested (if the concentration of the sample exceeds Standard curve range, please dilute appropriately and retest.
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Note: The above reagent quantities can be scaled proportionally based on the actual required total reaction volume (protein sample: reagent A: Folin phenol reagent=1:50:5)
Specifications & Purity
2N
Product Specifications
Concentration
2N
Storage Temp
Store at 2-8°C,Protected from light
Shipped In
Wet ice
Stability And Storage
Store at 4℃ in the dark for up to 1 year, or room temperature for up to 1 month.
Certificates
Certificate of Analysis(COA)
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K2429322