pAb; Rabbit anti Human GABARAPL1 Antibody; WB, IHC, Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104445-10μl | 10μl | In stock | $76.90 | |
| Ab104445-50μl | 50μl | In stock | $229.90 | |
| Ab104445-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 | |
| Ab104445-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human GABARAPL1 Antibody; WB, IHC, Flow; Unconjugated
| Product Name | GABARAPL1 Antibody - Primary antibody, specific to GABARAPL1, Rabbit IgG |
|---|---|
| Synonyms | APG8 like antibody; APG8L antibody; ATG8 antibody; ATG8B antibody; ATG8L antibody; Early estrogen regulated protein antibody; Early estrogen-regulated protein antibody; GABA antibody; GABA(A) receptor associated protein like 1 antibody; GABA(A) receptor-a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | GABARAPL1 |
| Immunogen | Recombinant human GABARAPL1 protein (AA 1-117). |
| Positive Control | WB: HeLa, HEK293, SK-N-SH, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human GABARAPL1 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
GABARAPL1 Antibody (Ab104445) - Western Blot
All lanes: GABARAPL1 Antibody (Ab104445) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16, 18 kDa
Exposure time: 100.0 s
GABARAPL1 Antibody (Ab104445) - Flow
GABARAPL1 Antibody (Ab104445) flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
GABARAPL1 Antibody (Ab104445) - IHC
Formalin-fixed and paraffin-embedded human brain tissue reacted with GABARAPL1 Antibody (Ab104445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/1000
|
| Flow | 1/10-1/50 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202932 | Certificate of Analysis | Mar 01, 2024 | Ab104445 |
| ZJ24F0202933 | Certificate of Analysis | Mar 01, 2024 | Ab104445 |
| 1. Kumar S et al.. (2018) Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins.. J Cell Biol, 217 (3): (997-1013). [PMID:29420192] |
| 2. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 3. Vernier-Magnin, S S and 9 more authors.. (2001) A novel early estrogen-regulated gene gec1 encodes a protein related to GABARAP.. Biochemical and biophysical research communications, (1): [PMID:11374880] |
| 4. Xin, Y Y and 8 more authors.. (2001) Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein.. Genomics, (15): [PMID:11414770] |
| 5. Chen, Chongguang C and 5 more authors.. (2006) GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor.. The Journal of biological chemistry, (24): [PMID:16431922] |
| 6. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 7. Pankiv, Serhiy S and 8 more authors.. (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy.. The Journal of biological chemistry, (17): [PMID:17580304] |
| 8. Behrends, Christian C, Sowa, Mathew E ME, Gygi, Steven P SP and Harper, J Wade JW.. (2010) Network organization of the human autophagy system.. Nature, (1): [PMID:20562859] |
| 9. Popovic, Doris D and 5 more authors.. (2012) Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.. Molecular and cellular biology, [PMID:22354992] |
| 10. Sancho, Ana A and 13 more authors.. (2012) DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription.. PloS one, [PMID:22470510] |
| 11. Colecchia, David D and 8 more authors.. (2012) MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins.. Autophagy, [PMID:22948227] |
| 12. Alemu, Endalkachew Ashenafi EA and 9 more authors.. (2012) ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs.. The Journal of biological chemistry, (16): [PMID:23043107] |
| 13. Choy, Augustine A and 6 more authors.. (2012) The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation.. Science (New York, N.Y.), (23): [PMID:23112293] |
| 14. Le Grand, Jaclyn Nicole JN and 7 more authors.. (2013) Specific distribution of the autophagic protein GABARAPL1/GEC1 in the developing and adult mouse brain and identification of neuronal populations expressing GABARAPL1/GEC1.. PloS one, [PMID:23690988] |
| 15. Lystad, Alf Håkon AH and 11 more authors.. (2014) Structural determinants in GABARAP required for the selective binding and recruitment of ALFY to LC3B-positive structures.. EMBO reports, [PMID:24668264] |
| 16. Mandell, Michael A MA and 13 more authors.. (2014) TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.. Developmental cell, (25): [PMID:25127057] |
| 17. Genau, Heide Marika HM and 7 more authors.. (2015) CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.. Molecular cell, (19): [PMID:25684205] |
| 18. Kimura, Tomonori T and 6 more authors.. (2015) TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.. The Journal of cell biology, (14): [PMID:26347139] |
| 19. Habisov, Sabrina S and 12 more authors.. (2016) Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation.. The Journal of biological chemistry, (22): [PMID:26929408] |
| 20. Skytte Rasmussen, Mads M and 11 more authors.. (2017) ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.. Autophagy, (4): [PMID:28287329] |
