Rabbit anti Human Galectin 9/Gal-9 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 IF/ICC: 1/200-1/1000 Protein Function Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Galectin 9/Gal-9 Antibody (Ab104612) - Western Blot All lanes: Galectin 9/Gal-9 Antibody (Ab104612) at 1/1000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 25.0 s
Galectin 9/Gal-9 Antibody (Ab104612) - IHC Immunohistochemical analysis of paraffin-embedded human tonsil. Galectin 9/Gal-9 Antibody (Ab104612) was diluted at 1/200 (4℃, overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
Galectin 9/Gal-9 Antibody (Ab104612) - IF/ICC Immunofluorescence analysis of HeLa cells. Galectin 9/Gal-9 Antibody (Ab104612) (green) was diluted at 1/200 (4℃, overnight). Goat Anti Rabbit Alexa Fluor 488 was diluted at 1/1000 (room temperature, 50 min). DAPI (blue) 10 min.
Galectin 9/Gal-9 Antibody (Ab104612) - IF/ICC Immunofluorescence analysis of NIH/3T3 cells, using Galectin 9/Gal-9 Antibody (Ab104612). The picture on the right is blocked with the synthesized peptide.