Ganoderic acid D, a highly oxygenated tetracyclic triterpenoid, is the major active component of Ganoderma lucidum . Ganoderic acid D upregulates the protein expression of SIRT3 and induces the deacetylated cyclophilin D (CypD) by SIRT3 . Ganoderic acid D inhibits the energy reprogramming of colon cancer cells including glucose uptake, lactate production, pyruvate and acetyl-coenzyme production in colon cancer cells Ganoderic acid D induces HeLa human cervical carcinoma apoptosis .
In Vitro
Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC 50 of 17.3 mM.\nGanoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells. Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 1, 5, 10, 20, 50 μM Incubation Time: 24, 48, 72 hours Result: Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. Cell Cycle AnalysisCell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10, 50 μM Incubation Time: 24, 48 hours Result: Induced G2/M phase arrest. Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. Apoptosis AnalysisCell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10, 50 μM Incubation Time: 48 hours Result: Induced a morphological change typical of apoptosis in HeLa cells. Western Blot AnalysisCell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10 μM Incubation Time: 48 hours Result: Up-regulated 14-3-3E and PRDX3.
