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GFP Mouse mAb - Primary antibody, specific to GFP, Mouse IgG1

1.0 mg/mL

Features and benefits

Short Overview:
mAb (3A10); Mouse anti GFP Antibody; WB, IP; Unconjugated
Species reactivity(Reacts with): Species independent
Isotype: Mouse IgG1

Application:

IF/ICC
WB

Item Number

Ab105284

Grouped product items
SKU	Size	Availability	Price
Ab105284-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$36.90
Ab105284-50μl	50μl	In stock	$109.90
Ab105284-100μl	100μl	In stock	$169.90
Ab105284-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,099.90

mAb (3A10); Mouse anti GFP Antibody; WB, IP; Unconjugated

View related series

GFP Tag

Basic Description

Product Name	GFP Mouse mAb - Primary antibody, specific to GFP, Mouse IgG1
Synonyms	GFP antibody; Green fluorescent protein antibody; eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein; GFP Tag antibody; Anti- GFP Tag antibody
Specifications & Purity	ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL
Host species	Mouse
Specificity	GFP
Immunogen	Synthetic peptide GFP.
Positive Control	WB: HEK293T whole cell lysate transfected with pEGFP-C3-ATG3, HEK293T whole cell lysate transfected with pEGFP-C3-ATG12. IP: HEK293T transfected with pEGFP-C3-ATG3#PPL00361-2a.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, High Performance, Validated
Product Description	Mouse anti GFP Antibody, Monoclonal (3A10), could be used for WB, IP and so on. Application: WB: 1/5000 - 1/10000 IF/ICC: 1/100 - 1/500 Relevance Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Biophysicochemical properties: Absorption: Abs(max)=395 nm Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

Product Properties

Isotype	Mouse IgG1
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa
Exposure time: 2.3 s

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Recombinant GFP Protein (rp156636) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 24.3 s

Application

Dilution info

1/5000 – 1/10000

IF/ICC

1/100-1/500

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1101580	Certificate of Analysis	Jul 30, 2024	Ab105284
ZJ23F1101581	Certificate of Analysis	Jul 30, 2024	Ab105284
ZJ24F0607569	Certificate of Analysis	Jun 28, 2024	Ab105284

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